Method for identifying rape honey and vervain family honey
A technology of chattia honey and rapeseed honey, which is applied in the field of identification of rapeseed honey and chattia honey, can solve the problems of complex operation, high dependence on identification result model establishment, and high identification cost
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[0028] Embodiment one: the identification method of rapeseed honey and twig honey, comprises the following steps:
[0029] 1) Extraction and hydrolysis of honey protein: Accurately weigh 10.0g (accurate to 0.01g) of honey sample into a 50mL centrifuge tube, add 20mL of water, mix well, and centrifuge at 1500g at 4°C for 10min to remove the pollen grains at the bottom. The supernatant was transferred to another centrifuge tube. Add 4 mL of 100% trichloroacetic acid to the supernatant, mix thoroughly, and place at -20°C for 15 minutes, then place at 4°C for 1 hour to precipitate the protein in honey. Centrifuge the centrifuge tube at 4°C, 10000g for 10min, discard the supernatant, and transfer the protein precipitate at the bottom layer to the hydrolysis tube with 3mL, 3mL and 4mL of 6.0mol / L hydrochloric acid solution three times, and fill it with nitrogen Finally, use a vacuum pump to vacuum, seal the hydrolysis tube and hydrolyze it in a constant temperature drying oven at 1...
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