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A creatinine detection reagent

A technology for detecting reagents and creatinine, which is applied in the field of medical testing, can solve the problems of lack of testing professionals and insufficient facilities for fully automatic biochemical testing equipment, and achieve good sensitivity, low price, and easy operation

Active Publication Date: 2015-09-02
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] With the "Eleventh Five-Year" plan for the purchase and strengthening of basic equipment for tertiary and secondary hospitals, hospitals above the middle level have good hardware and software facilities, but grassroots medical institutions, especially health centers, clinics and other units are not fully automatic. Biochemical testing equipment and facilities are generally insufficient, and there is not a sufficient number of testing professionals. Therefore, the establishment of a POCT analysis system that is easy to operate, low in price, and real-time reporting is of great significance to the role of grassroots hospitals in disease prevention and diagnosis and treatment.

Method used

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  • A creatinine detection reagent
  • A creatinine detection reagent
  • A creatinine detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Diluent:

[0042] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer (pH6.0) 0.1mol / L

[0043] Tween80 5%

[0044] Proclin300 0.1%

[0045] Bilirubin oxidase 1KU / L

[0046] Vitamin C oxidase 1KU / L

[0047] Reagent:

[0048] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer solution (pH7.0) 0.1mol / L

[0049] Creatinase 1KU / L

[0050] Sarcosine oxidase 1KU / L

[0051] Peroxidase (POD) 1KU / L

[0052] N-ethyl-N-(3-propanesulfo)-3-methylaniline (ESPMT) 0.1mmol / L

[0053] Creatinase 1KU / L

[0054] 4-aminoaminotipyrine (4-AAP) 0.1mmol / L

[0055] Sodium Benzoate 0.1g / L

[0056] Trehalose 10g / L

Embodiment 2

[0058] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer solution (pH7.0) 0.5mol / L

[0059] SPAN20 8%

[0060] Methylparaben 1%

[0061] Potassium ferrocyanide 1mol / L

[0062] Vitamin C oxidase 2KU / L

[0063] Reagent:

[0064] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer solution (pH7.8) 0.5mol / L

[0065] Creatinase 50KU / L

[0066] Sarcosine oxidase 50KU / L

[0067] Peroxidase (POD) 50KU / L

[0068] N-ethyl-N-(3-propanesulfonyl)-3-methylaniline (ESPMT) 50mmol / L

[0069] Creatinase 150KU / L

[0070] 4-Aminoaminotipyrine (4-AAP) 50mmol / L

[0071] Proclin300 1g / L

[0072] Bovine serum albumin 10g / L

Embodiment 3

[0074] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer solution (pH8.5) 1.0mol / L

[0075] Triton100 5%

[0076] Potassium sorbate 1%

[0077] Potassium ferricyanide 10mol / L

[0078] Vitamin C oxidase 10KU / L

[0079] Reagent:

[0080] N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer solution (pH8.5) 1.0mol / L

[0081] Creatinase 100KU / L

[0082] Sarcosine oxidase 100KU / L

[0083] Peroxidase (POD) 100KU / L

[0084] N-ethyl-N-(3-propanesulfonyl)-3-methylaniline (ESPMT) 100mmol / L

[0085] Creatinase 300KU / L

[0086] 4-Aminoaminotipyrine (4-AAP) 100mmol / L

[0087] Sodium nitrite 1g / L

[0088] Fatty alcohol polyoxyethylene ether 10g / L.

[0089] The performance of the reagent obtained in Example 1 of the present invention is described below in conjunction with the table.

[0090] 1. Precision

[0091] Table 1. Precision evaluation results

[0092]

[0093] 2. Linear

[0094] The standard serum sample is added with different concentrations of...

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Abstract

The invention discloses a creatinine detection reagent which is characterized by comprising a diluent and a reaction reagent, wherein the diluent is composed of buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of buffer, creatinase, sarcosine oxidase, peroxidase, N-ethyl-N-(3-propylsulfo)-3-methylaniline, creatininase, 4-aminoantipyrine, preservative and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a creatinine detection reagent used in a POCT analyzer. Background technique [0002] Creatinine (CR) is a product of human muscle metabolism. It is mainly formed slowly by creatine through an irreversible non-enzymatic dehydration reaction, and then released into the blood, excreted with urine, not easily affected by diet, and can be filtered through the glomerulus. It is rarely reabsorbed in tubules. Creatinine includes blood creatinine and urine creatinine. Serum creatinine is more meaningful to measure renal function. Serum creatinine concentration is an effective indicator for evaluating glomerular filtration rate, which can effectively reflect the substantial damage of renal function It is of great significance to detect clinically to help judge the condition. Picric acid method (Jaffe's method) and enzyme method are commonly used in CR clinically. Although Jaffe's...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 邹炳德邹继华沃燕波张桂春
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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