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Receptor kinase albuminoid of rice brassinosteroid and coding genes and applications thereof

A technology of brassinolide and receptor kinase, which is applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve problems such as segregated family genes that have not yet been seen, and achieve the effect of broad application prospects

Inactive Publication Date: 2013-08-28
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many members of the OsSERKs family in rice, but there is no report on the isolation of genes of this family with definite disease resistance functions from rice

Method used

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  • Receptor kinase albuminoid of rice brassinosteroid and coding genes and applications thereof
  • Receptor kinase albuminoid of rice brassinosteroid and coding genes and applications thereof
  • Receptor kinase albuminoid of rice brassinosteroid and coding genes and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1. Obtainment of Rice Brassinolide Receptor Kinase-like Protein OsBAK1L and Its Encoding Gene

[0039] According to the rice gene (LOC_Os03g49620.4 sequence, use Primer Premicr5.00 to design a pair of primers OEBF and OEBR, OEBF sequence is: 5'ATGGGGAGGTGCCATACTGG3', OEBR is 5'TOTGCCGGCGGAGAGCT3'. Use the above primers for RT-PCR. With 200mg of rice variety 9804 The blade of -Rxol is material, adopts Trizol method to extract total RNA, detects the completeness of RNA through 1% agarose electrophoresis.The synthesis of Ss cDNA is according to the method for SuperScript TM II RNase H-ReverseTranscriptase, synthesizes single strand (ss) cDNA. The RT-PCR reaction program is as follows: first 94°C for 5 minutes; then 30 cycles (94°C for 30 seconds, 52°C for 1 minute, 72°C for 2 minutes); finally 72°C for 15 minutes, and 4°C for incubation. The PCR product was 1% Agarose gel electrophoresis detection. The result of amplification is as follows figure 1 As shown, the O...

Embodiment 2

[0044] Example 2 Obtaining and identification of disease resistance of OsBAK1L transgenic rice

[0045] 1. Construction of OsBAK1L plant RNAi expression vector

[0046] 根据NCBI在线BLAST对OsBAK1L同源序列分析,选择其中540bp特异核苷酸序列(GGTGAGGAGGTGAAGTGGGAGAGGCGGCGTGGACCTGCGACGACCTGCTTGCTGCCCAGCCTGCGAGGGGCAAGCCACTGGTAGGTGCCATACTGGACTGGCTTGCTTGTTAACTGGGGTTAGCCAGGGGACTCCGGTATTATACTACTATGATCAGTTCGTGTACTTTTTTTCGCCGGGGCAAGTTTCCCCAAGGAACTCTTTTGATGCTGTAATTATAACTGAAGAGTCATGGATCTGCTCAGCGTCCTCCTAATTATAGCATCTCTGCTCCCATTTTCAGCATCTGACCGTCAAGGTGATGCACTATATGATATGAAGCTGAAGCTGAATGCTACTGGTAACCAGCTTTCTGACTGGAACCAAAATCAAGTTAACCCATGCACTTGGAATTCTGTTATTTGTGACAACAACTACAATGTTGTGCAAGTAACATTGGCATCTATGGGATTCACTGGAGTTCTATCACCACGAATTGGAGAGCTTCAGTTTTTGAATGTTTTGTCCTTGCCTGGTAATAAGATTACTGGTGGCATACCTGAGC)作为RNAi干扰区段。 Primers RiBF / R were designed, the sequence of RiBF was: 5'GGTGAGGAGGTGAAGTGGGA3', and the sequence of RiBR was: 5'GCTCAGGTATGCCACCAGTAA3'. PrimeSTARPCR amplifies the OsBAK1L-specific 540bp segment, and then uses PrimeSTA...

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Abstract

The invention discloses a receptor kinase albuminoid of rice brassinosteroid and coding genes and applications thereof. Proteins related to the disease resistance of plants as provided in the invention have proteins which contain one of following amino acid residue sequences: 1) sequence of SEQ ID No1 in the sequence table; 2) proteins related to the disease resistance of plants and containing a SEQ ID No: 1 amino acid residue sequence in the sequence table with replacement, deletion and addition of 1-10 amino acid residues. After being inoculated with pathogenic bacteria, proteins in the invention can induce the up-regulation expression in plants which carry non-host resistance genes and can increase the plant disease resistance.

Description

technical field [0001] The present invention relates to a plant disease resistance-related protein and its coding gene and application, in particular to a rice-derived brassinosteroid (Brassinosteroids, BRs) receptor kinase analog protein (BRI1-associated kinasc1, Bak1) and its coding Genes and their application in improving plant disease resistance. Background technique [0002] Diseases are one of the important limiting factors for high and stable crop yields. Breeding and planting disease-resistant varieties is one of the most cost-effective measures to prevent and control plant diseases. Plant disease resistance genes include host disease resistance genes and non-host disease resistance genes. At present, the quality resistance of host plants controlled by a single disease resistance gene is mainly used in the breeding process. The reason is that this type of gene can produce a typical disease resistance response to a specific pathogen, and a single gene is easy to use...

Claims

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Application Information

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IPC IPC(8): C12N9/12C12N15/54C12N15/82C12N5/10C12N1/21C12N1/15C12N1/19A01H5/00
Inventor 周永力陈现朝黄立钰张帆黎志康
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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