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Method for building mouse severe aplastic anemia model

An aplastic, mouse technology, applied in the direction of pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., can solve the problems of high technical requirements, high cost, instability, etc., and achieves low cost and easy operation. Effect

Inactive Publication Date: 2013-07-03
AFFILIATED HOSPITAL OF NANTONG UNIV
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Problems solved by technology

The modeling time period of the chemical method is long and unstable; the model of the physical method will damage the organs of the whole body; the hematopoietic function of the mixed method will return to normal on its own at about 25 days, so the model induced by physical or chemical factors is not suitable for aplastic disorders anemia research
However, the immune-mediated aplastic anemia model has good stability and is closer to the human immune-mediated bone marrow hematopoietic injury, but it requires high technical requirements and consumes a lot of money, making it difficult to carry out in ordinary laboratories.

Method used

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  • Method for building mouse severe aplastic anemia model
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Embodiment Construction

[0008] In order to deepen the understanding of the present invention, the present invention will be further described below in conjunction with the embodiments and accompanying drawings. The embodiments are only used to explain the present invention, and do not constitute a limitation to the protection scope of the present invention.

[0009] like figure 1 and figure 2 The result figure after the specific embodiment of the present invention is shown, the method for establishing a severe aplastic anemia model in mice, through the administration of interferon-γ combined with busulfan, using inbred female BALB / c mice as The model mice were given Busulfan 18mg / kg / d orally, and at the same time, intraperitoneally injected Interferon-γ7500U / d for 10 consecutive days. On the 10th day after the end of the administration, the prevalence rate of severe aplastic anemia in the mice was 100%, and the mortality rate was 20%. The white blood cell count of the model mice was (0.8±0.3)×10 ...

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Abstract

The invention discloses a method for building a mouse severe aplastic anemia model, wherein the mouse severe aplastic anemia model is built through combination of an interferon-gama with a busulfan administration method, and the mouse severe aplastic anemia model induced by the invention is simple and convenient in method and high in success rate of induction and accords with the characteristics of human aplastic anemia.

Description

technical field [0001] The invention relates to a method for a bone marrow failure disease model, in particular to a method for establishing a severe aplastic anemia model in mice. Background technique [0002] At present, most researchers at home and abroad use mice to establish mouse models of severe aplastic anemia to study the pathogenesis of aplastic anemia. The methods for inducing severe aplastic anemia mouse models mainly include chemical methods, physical methods, immune-mediated methods and mixed methods. The modeling time period of the chemical method is long and unstable; the model of the physical method will damage the organs of the whole body; the hematopoietic function of the mixed method will return to normal on its own at about 25 days, so the model induced by physical or chemical factors is not suitable for aplastic disorders Anemia research. The immune-mediated aplastic anemia model has good stability and is closer to the human immune-mediated bone marro...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/19A61K31/255
Inventor 刘红林赠华
Owner AFFILIATED HOSPITAL OF NANTONG UNIV
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