Precursor culture medium for wild dendrobium candidum

Abstract

The invention relates to a precursor culture medium for wild dendrobium candidum. A composition formula of the culture medium is formed from the following raw materials by weight: a B5 culture medium, 2.2 mg / L of naphthylacetic acid, 2.5 mg / L of indolebutyric acid, 16 percent of banana extract, 15 percent of potato extract, 2.6 percent of sucrose, 0.65 percent of agar and 0.5 percent of potassium. By regulating the dendrobium candidum culture medium, effective accumulation of secondary metabolites of a new variety of test-tube plantlet of dendrobium candidum is promoted to be increased; and due to change of precursor culture conditions, the aim of industrial production of the wild dendrobium candidum is fulfilled. Active ingredients of a test-tube plantlet of dendrobium candidum, which grows for 7 to 8 months, are superior to those of triennial wild seedlings of dendrobium candidum, the growth cycle of dendrobium candidum is shortened, the planting manner of dendrobium candidum is changed, a land planting area is greatly saved, production cost is reduced, and necessary conditions are provided for comprehensive utilization and deep processing of the wild dendrobium candidum.

Description

technical field [0001] The invention relates to a culture medium, in particular to a precursor culture medium of wild Dendrobium officinale. Background technique [0002] Dendrobium officinale is a rare and endangered medicinal material with high added value. It is recorded in the classics. In the first year of Tang Kaiyuan, the Taoist classic "Tao Zang" included nine Chinese immortal grasses (Dendrobium officinale, Tianshan snow lotus, Sanliang ginseng, 120-year-old polygoni multiflorum, sixty-year-old Poria cocos, cistanche, deep mountain ganoderma, sea pearl, and cordyceps sinensis). Dendrobium officinale has a remarkable effect of nourishing yin and tonifying deficiency, and is listed as the first of the "Nine Chinese Grass Immortals". Li Shizhen's "Compendium of Materia Medica" records: "Strengthening yin and benefiting essence. Take it for a long time, thicken the stomach, nourish the internal deficiency, calm stomach qi, grow muscles, eliminate skin evil heat and pr...

AI Technical Summary

Problems solved by technology

Since ancient times, people have been exploring the artificial cultivation of Dendrobium, but due to the low reproduction coefficient of Dendrobium plants, the annual output is limited, and it takes a long time to enter the peak production period, so the scale cannot be formed, and the output is extremely limited.

At present, the low survival rate of transplanted tissue culture seedlings is common, how to improve the survival rate of Dendrobium officinale cultivation has become a major obstacle restricting the production and development of Dendrobium