Kit for auxiliary diagnosis of squamous cell lung carcinoma patient

A technology for auxiliary diagnosis and squamous cell carcinoma of the lung, applied in biological testing, material inspection products, measuring devices, etc., can solve problems such as low diagnostic efficiency and failure to meet clinical needs, and achieve high sensitivity and strong specificity

Active Publication Date: 2013-06-26
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Although the above protein markers have been used in the auxiliary diagnosis of lung cancer, their diagnostic efficiency is low and cannot meet the clinical needs

Method used

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  • Kit for auxiliary diagnosis of squamous cell lung carcinoma patient
  • Kit for auxiliary diagnosis of squamous cell lung carcinoma patient
  • Kit for auxiliary diagnosis of squamous cell lung carcinoma patient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, establishment of the inventive method

[0033] 1. Model establishment

[0034]A total of 489 plasma samples from patients with squamous cell carcinoma of the lung and 479 plasma samples from healthy people (normal controls) were collected from lung cancer patients diagnosed by pathology in the Department of Thoracic Surgery, Cancer Hospital, Chinese Academy of Medical Sciences, and normal people who participated in cancer screening. This experiment was approved by the Ethics Committee, and all subjects were informed and signed an informed consent form. Among patients with squamous cell carcinoma of the lung, there were 466 males and 23 females, with an age range of 28-86 years, with an average age of 61.56 years; among the normal population, there were 240 males and 239 females, with an age range of 41-70 years, with an average age of 61.56 years. For 57.39 years. Plasma samples were obtained before surgery, and the patients had not received chemotherapy...

Embodiment 2

[0045] Embodiment 2, verification of the inventive method

[0046] The patients used in this example were 150 patients with clinically diagnosed squamous cell carcinoma of the lung (peripheral venous blood was obtained before surgery, and the patients had not received chemotherapy, radiotherapy, intervention, etc.) and 50 healthy people (non-squamous cell carcinoma of the lung) patients), all volunteers who gave informed consent.

[0047] 1. Take 5ml of peripheral venous blood from the patient to be tested on an empty stomach, let it stand at room temperature for 1h, then centrifuge at 3000r / min for 15min, take the supernatant, divide it into two 1.5mL EP tubes, and store in a -80°C refrigerator.

[0048] 2. Dissolve the sample from step 1 at room temperature for 3 hours, then centrifuge at 3000r / min for 5 minutes.

[0049] 3. Detect the concentration of the protein marker IDH1 (in U / L), the concentration of the protein marker CA125 (in U / mL), and the concentration of the pro...

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Abstract

The invention discloses a kit for auxiliary diagnosis of squamous cell lung carcinoma patient. The kit provided by the invention comprises a product for detecting a protein marker IDH1, a product for detecting a protein marker CA125, a product for detecting a protein marker CYFRA21-1 and a carrier recorded with the following functional expressions (as shown in the description): x1 stands for the concentration of the IDH1, x2 stands for the concentration of the CA125, and x3 stands for the concentration of the CYFRA21-1. The kit provided by the invention is used for the auxiliary diagnosis of squamous cell lung carcinoma patient according to the corresponding diagnosis standard, and has the characteristics of high flexibility and strong specificity, the diagnosis result reliability is far better than the diagnosis by adopting the single protein marker, and has important value and application prospect for the diagnosis and treatment of the squamous cell lung carcinoma.

Description

technical field [0001] The invention relates to a kit for assisting diagnosis of lung squamous cell carcinoma patients. Background technique [0002] Lung cancer is a major disease that seriously threatens human life and health. In 2011, the World Health Organization (WHO) International Cancer Research Agency IARC released the results of 2008 global statistical data, showing that lung cancer is the malignant tumor with the highest incidence and mortality. Every year, there are 1.608 million new cases and 1.377 million deaths worldwide, accounting for 12.7% and 18.2% of all malignant tumors. According to statistics released by the American Cancer Society, it is estimated that in 2012, the number of new lung cancer cases in the United States will reach 226,000, and the number of deaths due to lung cancer will reach 160,000. In my country, the results of the third national retrospective sampling survey on causes of death hosted by the Ministry of Health show that lung cancer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
Inventor 赫捷谭锋维孙楠陈照丽
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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