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Detection kit for detecting and distinguishing multiple kinds of echinococcus

A detection kit and a technology for Echinococcus multilocularis, which are applied in the field of detection kits for detecting and distinguishing various Echinococcus tapeworms, can solve the problem of typing detection of various Echinococcus tapeworms, waste of materials, cumbersome detection process, etc. To achieve the effect of high specificity of test results, rapid detection, and sensitive detection response

Active Publication Date: 2014-06-11
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Among the above methods, the conventional detection method has the risk of infecting people and the surrounding environment, and the other methods cannot perform typing detection of multiple echinococcus tapeworms in one reaction, and there are problems of cumbersome detection process and waste of materials

Method used

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  • Detection kit for detecting and distinguishing multiple kinds of echinococcus
  • Detection kit for detecting and distinguishing multiple kinds of echinococcus
  • Detection kit for detecting and distinguishing multiple kinds of echinococcus

Examples

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Embodiment Construction

[0015] In this example, feces from stray dogs were selected for detection of echinococcosis infection. Take an appropriate amount of stray dog ​​feces, float the eggs and body fragments in the dog feces with saturated salt water flotation method, collect by centrifugation, and use the fecal DNA extraction kit to extract the whole genome DNA, measure the concentration of the extracted DNA for later use. Prepare a compound PCR reaction solution in a PCR reaction tube, using a 50 μL reaction system, including 5 μL of 10×PCR Buffer, MgCl 2 (25 mmol / μL) 5 μL of upstream primer F1 for specific detection of Echinococcus granulosus: 5'- GGTTTTATCGGTATGTTGGTGTTAGTG-3' (SEQ ID №1) 1 μL (50 μmol / mL) and downstream primer R1: 5'- CATTTCYTGAAGTTAACAGCATCACG-3' (SEQ ID №2) 1 μL (50 μmol / mL), upstream primer F2 for specific detection of Echinococcus multilocularis: 5'- CATTAATTATGGATGTTTCC-3' (SEQ ID №3) 1 μL (50 μmol / mL) and downstream primer R2: 5'-GGAAATACCCCACTATCC-3' (SEQ ID №4) 1 μL (...

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Abstract

The invention discloses a kit for detecting echinococcus granulosus, echinococcus multilocularis and echinococcus shiquicus. Three pairs of specific amplification primers aiming at three pathogens are respectively arranged in the detection kit. By utilizing the three pairs of primers in the kit, a composite polymerase chain reaction (PCR) method can be used for detecting independent or mixed infection of the three pathogens, and whether the detected sample is infected with echinococcus can be determined according to specific strips occurring in electrophoresis of amplification products.

Description

technical field [0001] The invention relates to a detection kit for detecting and distinguishing Echinococcus granulosus, Echinococcus multilocularis and Echinococcus shiqu. Background technique [0002] Echinococcosis, commonly known as hydatid disease, is a zoonotic parasitic disease caused by the larvae of Echinococcus tapeworm—hydatid cyst. Echinococcus granulosus (Echinococcus granulosus) Echinococcus granulosus , Eg), Echinococcus multilocularis ( Echinococcus multilocularis , Em), Echinococcus fowleri ( Echinococcus vogeli , Ev), Echinococcus oligoarthritis ( Echinococcus oligarthus , Eo) and Echinococcus shiqu ( Echinococcus shaquacus , Es). The larvae of Echinococcus granulosus, Echinococcus multilocularis, and Echinococcus fowleri cause cystic echinococcosis, alveolar echinococcosis, and polycystic echinococcosis, respectively. Coccidiosis has caused great harm to human health and seriously hindered the development of animal husbandry. In China, there ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 娄忠子贾万忠闫鸿斌李宏民李立范彦雷倪兴维
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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