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Kit for detecting HLA-B*1301 gene

An HLA-B and kit technology, which is applied in the determination/inspection of microorganisms, fluorescence/phosphorescence, biochemical equipment and methods, etc., can solve the problems of affecting the expression of cell surface antigens, cumbersome steps, and time-consuming

Active Publication Date: 2013-06-26
CHUGOKU IGAKU KAGAKUIN HIFUBIYOU KENKYUSHO
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method requires pretreatment of the test sample, but the difference in the treatment method or time may sometimes affect the expression of cell surface antigens, thereby affecting the judgment of the result
SSOP is a new type of DNA typing method in HLA, the result is accurate, but the steps are cumbersome and time-consuming

Method used

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  • Kit for detecting HLA-B*1301 gene
  • Kit for detecting HLA-B*1301 gene
  • Kit for detecting HLA-B*1301 gene

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Embodiment Construction

[0017] 1. Experimental method

[0018] 1. Patient

[0019] A total of 1058 positive leprosy patients were included in this study from June 2010 to June 2012 in Southwest China. At each visit, a clinician recorded the patient's treatment history and details of adverse drug reactions. Using standard diagnostic criteria, the medical records of individuals with DDS were reviewed by a clinician (Yan LB). Clinical morphology according to the diagnostic criteria of Richardus and Smith: (i) at least two signs or symptoms, such as fever, rash, lymphadenopathy, or liver abnormalities (hepatomegaly, jaundice, or liver function test confusion); (ii) Symptoms appeared between two and eight weeks after initiation of dapsone treatment and disappeared after discontinuation; (iii) symptoms were not attributable to irritation by any other drug; (iv) symptoms did not cause a leprosy reaction; (v) no other disease could have caused Similar symptoms.

[0020] 21 cases of DHS, 110 DDS-tolerant ...

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Abstract

The invention discloses a kit for detecting HLA-B*1301 gene. The kit comprises the following components: 2*PCR Mix, a target gene detection mix, a reference genes detection mix, a positive reference group pMED-B*1301 and deionized water, wherein the gene detection mix comprises 5'-ggtctcacatcatccagagg-3', and a downstream primer 5'ttcctctgcgacgtcgcg-3'; and the reference genes detection mix comprises an upstream primer beta-F:5'- ctgggacgacatggagaaaa-3', and a downstream primer beta-R:5'- aaggaaggctggaagagtgc-3'. As HLA-B*1301 has close relationship with DHS and has the potential to be the biomarker for predicting dapsone allergic syndrome morbidity of Chinese people, by researching and developing the kit to detect the prediction marker HLA-B*1301 of the dapsone allergic syndrome morbidity, the dapsone allergic syndrome morbidity of leprosy patients can be monitored, therefore, the kit has important clinical significance.

Description

technical field [0001] The invention relates to a kit for SYBR Green detection of HLA-B*1301 gene. Background technique [0002] Leprosy, caused by Mycobacterium leprae, is a chronic disease characterized by slowly progressive damage to peripheral nerves and skin that, if left untreated, can lead to deformity and debilitation. The World Health Organization (WHO) reported that in early 2011, 192,246 cases of leprosy began to be registered globally, and in 2010, 228,474 new cases were discovered worldwide. Since 1949, more than 490,000 leprosy patients have been diagnosed in my country, and in the most recent period, about 1,800 new cases were reported each year. [0003] In the 1940s, scientists discovered that dapsone (4,40-diaminodipheny-lsulfone, DDS) could effectively treat leprosy. However, in the 1960s, Mycobacterium leprae became resistant to this. Dapsone hypersensitivity syndrome (DHS) was first diagnosed in 1949, its symptoms include fever, exfoliative dermatitis...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 王宝玺王洪生
Owner CHUGOKU IGAKU KAGAKUIN HIFUBIYOU KENKYUSHO
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