Method for preparing effective component of mottle clam health-care product with hypoglycemic effect
A technology for active ingredients and variegated clams, which is applied in the field of preparation of active ingredients of health products, can solve the problems of obtaining AGD inhibitors and the like, and achieves the effects of good inhibitory activity, wide sources and low price.
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Embodiment 1
[0016] Take fresh and alive variegated clams, wash and take the meat, mash through a tissue masher to obtain variegated clam meat, add 20 times the mass of deionized water to the meat mince and mix evenly to obtain clam meat homogenate, add clam meat homogenate Add 2% pepsin (enzyme activity unit: 1:3000) of variegated clam meat to the mixture, adjust the pH value of the mixture to 2.0, and enzymolyze it for 2 hours at a temperature of 37°C to obtain variegated clam meat enzymatic hydrolysis solution; the above-mentioned variegated clam meat enzymatic solution was heated to 95°C, kept for 10 minutes, and the pH was adjusted to 7.0 with NaOH to obtain the clam meat enzyme solution; the clam meat enzyme solution was centrifuged at 5000 rpm for 10 Minutes, and then take the supernatant; freeze-dry the supernatant to obtain the active ingredients of the solid nutritional health product.
[0017] According to in vitro experiments, the inhibitory rate of this active ingredient on α-...
Embodiment 2
[0019] Take fresh and alive variegated clams, wash and take the meat, mash through a tissue masher to obtain variegated clam meat, add 15 times the mass of deionized water to the meat mince and mix evenly to obtain clam meat homogenate, and add clam meat homogenate Add pepsin with 1.5% of the mass of variegated clam meat (enzyme activity unit is 1:3000), adjust the pH value of the mixture to 2.0, and enzymatically hydrolyze it for 2.5 hours at a temperature of 37°C to obtain variegated clam meat enzymatic hydrolysis solution; heat the above-mentioned variegated clam meat enzymatic solution to 100 ° C, keep it for 10 minutes, adjust the pH to 7.0 with NaOH, and obtain the clam meat enzyme solution; centrifuge the clam meat enzyme solution at 4000 rpm for 15 minutes, and then take the supernatant; spray-dry the supernatant to obtain the active ingredients of solid nutrition and health products.
[0020] According to in vitro experiments, the inhibitory rate of this active ingred...
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