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Method of measuring viscoelasticity coefficients of material and device for measuring viscoelasticity coefficients of material

A measurement method and viscoelasticity technology, which is applied in the measurement device, the use of material absorption and weighing, flow characteristics, etc.

Active Publication Date: 2013-05-15
ULVAC INC
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0007] However, even if the mass can be converted from the frequency change caused by the separated mass load using the aforementioned Sauerbrey formula, the viscoelastic coefficient of the adsorbed substance cannot be calculated from the separated viscoelastic elements.
[0008] Specifically, according to the invention described in Patent Document 1, it has been found that the F contained in the above-mentioned s The three elements in the frequency change are calculated separately as the frequency change. However, the mass load term, viscous load term, and viscoelastic term cannot be separated from each other in all measurements. Even if there is no viscous change, viscous Variations are not zero and are computed
In addition, the separated items are frequency values, and the viscoelastic coefficient cannot be calculated

Method used

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  • Method of measuring viscoelasticity coefficients of material and device for measuring viscoelasticity coefficients of material
  • Method of measuring viscoelasticity coefficients of material and device for measuring viscoelasticity coefficients of material
  • Method of measuring viscoelasticity coefficients of material and device for measuring viscoelasticity coefficients of material

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Embodiment

[0119] Use refer to the above figure 1 In the described apparatus, the results of obtaining the viscoelastic coefficients of biomolecular membranes and the like shown in Table 1 below using the fundamental wave and the triple wave of 27 MHz in the present invention are shown in the table.

[0120] In addition, each sample used in the experiment is as follows.

[0121] 1) 50nm beads (final concentration when assayed under latex beads is 10 μg / ml)

[0122] 2) Protein (Neutravidin) (final concentration when measured under globular protein is 10 μg / ml)

[0123] 3) ssDNA30mer (30-mer of single-stranded DNA, the final concentration during measurement is 1 μg / ml)

[0124] 4) ssDNA60mer (60-mer of single-stranded DNA, the final concentration of the assay is 1 μg / ml)

[0125] 4) ssDNA90mer (90-mer of single-stranded DNA, the final concentration of the assay is 1 μg / ml)

[0126] 5) dsDNA90mer (90-mer of double-stranded DNA, the final concentration of the assay is 1 μg / ml)

[0127] ...

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Abstract

To provide a method of measuring the viscoelasticity coefficients of a material and a device for the viscoelasticity coefficients of a material with which it is possible to represent information of the viscoelasticity of an adsorbent material with coefficients G and G which are typically used in representing viscoelasticity, and with which it is further possible to compute the viscoelasticity coefficients in real time. In a system wherein a material is adsorbed and a film formed, either upon the surface of a piezoelectric element in solution, or on a film which is fixed upon the piezoelectric element in solution, using at least two of N overtones of the piezoelectric element, and for each N overtone, employing at least two of the resonant frequency FS and half-value frequencies F1, F2 (where F2 > F1) having half the conductance value of the conductance value of the resonant frequency, the mass load term, the viscoelasticity term, the viscoelasticity term, and the viscoelasticity term are computed, and the viscoelasticity coefficients G (stored modulus portion) and G (loss modulus portion) of the film are computed.

Description

technical field [0001] The present invention relates to a method for measuring the viscoelastic coefficient of a substance and a device for measuring the viscoelastic coefficient of a substance using a sensor in the fields of chemistry, physics, biochemistry, pharmacy, materials, etc. The sensor is used for the adsorption measurement of a substance in a solution and physical performance evaluation. Background technique [0002] The relationship between the frequency change of adsorption by QCM and the mass load uses Sauerbrey's equation shown in Mathematical Expression 14 below. [0003] [mathematical formula 14] [0004] ΔFs = - 2 N f 0 2 ρ Q μ Q Δm A ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N5/02G01N11/16
CPCG01N5/02G01N11/16G01N2291/0256G01N29/02G01N2291/0423G01N29/036G01N2291/02827G01N29/022G01N2291/0427G06F17/13G01N2291/0426G01N2203/0094
Inventor 伊藤敦市桥素子
Owner ULVAC INC
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