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Application of lysine as synergist for preparing antifungal drug

An antifungal drug, lysine technology, applied in the field of medicine, can solve problems such as missing lysine, and achieve the effects of reducing drug dosage and reducing toxic and side effects

Inactive Publication Date: 2014-06-04
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report on the use of lysine as a synergist of polyenes or echinocandins antifungal drugs

Method used

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  • Application of lysine as synergist for preparing antifungal drug
  • Application of lysine as synergist for preparing antifungal drug
  • Application of lysine as synergist for preparing antifungal drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1: Comparative experiment on the antibacterial effect of amphotericin B alone and in combination with lysine on different clinical fungal isolates

[0011] Materials and methods

[0012] 1. Test drug:

[0013] Lysine: American Sigma Reagent Company.

[0014] Amphotericin B: China Institute for the Control of Pharmaceutical and Biological Products (the same below).

[0015] Dimethyl sulfoxide: China National Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0016] The concentration of lysine was 40mM, the concentration of amphotericin B was 2mg / ml, and the tested drugs were stored at -20℃. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0017] 2. Strains:

[0018] The clinical strains of Candida albicans, Candida krusei and Microsporum lanoides were provided by the fungal laboratory of Shanghai Changhai Hospital, and were...

Embodiment 2

[0044] Embodiment 2: Nystatin is used alone and combined with lysine antifungal effect comparative experiment

[0045] Materials and methods

[0046] 1. Test drug:

[0047] Lysine: American Sigma Reagent Company.

[0048] Nystatin: American Sigma Reagent Company.

[0049] Dimethyl sulfoxide: China National Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0050] The concentration of lysine was 40mM, the concentration of nystatin was 2mg / ml, and the tested drugs were stored at -20℃. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0051] Other experimental steps and methods are the same as in Example 1.

[0052] The experimental results are shown in Table 3.

[0053] Table 3 MIC of nystatin alone and in combination with lysine on 5 strains of clinical Candida albicans 80 value

[0054]

[0055] It can be seen from Table 3 that lysine...

Embodiment 3

[0056] Embodiment 3: Caspofungin is used alone and combined with lysine antifungal effect comparative experiment

[0057] Materials and methods

[0058] 1. Test drug:

[0059] Lysine: American Sigma Reagent Company.

[0060]Caspofungin: American Merck Company.

[0061] Dimethyl sulfoxide: China National Pharmaceutical (Group) Shanghai Chemical Reagent Company.

[0062] The concentration of lysine was 40mM, the concentration of caspofungin was 2mg / ml, and the tested drugs were stored at -20℃. Before the experiment, the drug stock solution was taken out and placed in a 35°C incubator to melt, fully mixed, and the pharmacodynamic test was carried out respectively.

[0063] Other experimental steps and methods are the same as in Example 1.

[0064] The experimental results are shown in Table 4.

[0065] Table 4 Caspofungin alone and in combination with lysine to the MIC of 5 clinical Candida albicans 80 value

[0066]

[0067] It can be seen from Table 4 that lysine has...

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Abstract

The invention relates to the medical technology field, and discloses an application of lysine as a synergist for preparing antifungal drugs like polyenes or echinocandins. The antifungal drugs may be amphotericin B, nystatin or caspofungin. The amount of lysine or pharmaceutically acceptable salts thereof in the antifungal drug effective concentration is 0.03-0.5mM. As shown in an experiment, the combination application of antifungal drugs like amphotericin B, nystatin and caspofungin, can also ensure the treatment effect on superficial and deep fungal infections in case of decrease of drug dose, and the combination can be used as a synergist of the antifungal drugs. The lysine, as the synergist of the antifungal drugs, can decrease the medical dosage of the antifungal drugs like polyenes or echinocandins, thereby alleviating the toxic and side effect of medicaments, effectively treating fungal infection, and having a high application value.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to the application of lysine as a synergist in the preparation of antifungal drugs. Background technique [0002] In recent years, the clinical deep fungal infection rate has risen sharply, and it has become one of the main causes of death in immunocompromised patients. At present, there are few drugs available for clinical treatment of deep fungal infections, and the side effects are large. Coupled with the increasingly serious phenomenon of fungal drug resistance, the prevention and treatment has become a difficult clinical problem. Antifungal drugs such as polyenes (such as amphotericin B, nystatin) and echinocandins (such as caspofungin) are commonly used clinically and can effectively treat deep and superficial fungal infections. However, due to well-known reasons, clinical drug-resistant strains are becoming more and more common, resulting in the need to increase the dosage, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K31/198A61K38/12A61P31/10
Inventor 曹颖瑛赵柳娅高越朱臻宇姚响文杨宇柴逸峰姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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