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Artificial Chinese eaglewood forming agent

A fragrance, artificial technology, used in biocides, animal repellents, plant growth regulators, etc., can solve problems such as shortening the time of fragrance formation

Inactive Publication Date: 2013-04-10
CHANGZHOU ADAM BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the patent of the present invention is to provide an artificial agarwood fragrance-making agent and a preparation method for the disadvantages of the artificial agarwood fragrance-making method at the present stage, which can improve the fragrance-making rate of agarwood and shorten the fragrance-forming time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1. Dust particles in the surrounding radius 0.5-1m of the Arakita japonica: collection method: spread a layer of plastic film around the Arakina japonica, fill the PDA flat plate solid medium on the film, after 24h, place the flat plate Take it back, put it in a constant temperature incubator and cultivate it for 48 hours. After the colony grows on the plate, scrape out the place where there is a colony with an inoculation needle; insert it into the PDB medium, and cultivate it on a shaking table for 48 hours to obtain a bacterial suspension; the culture condition is : Inoculum size 1-2%, shaker speed 100-150rpm, 30-35°C. Potassium salt solution is 2% KCl solution. The preparation method is as follows: (1) after 48 hours of microbial culture, obtain the bacterial suspension; (2) mix the obtained microbial bacterial suspension with the potassium salt solution in proportion, that is, the volume ratio of the microorganism and the potassium salt solution is 1:2-...

Embodiment 2

[0013] Example 2 The soil in the radius of 0.5-1m around the trunk of A. chinensis, collection method: adopt the soil around the roots of A. chinensis. After the water evaporates to dryness, put the soil into a conical flask equipped with sterile water, and add Shake the sterilized glass beads for 30 minutes to effectively separate the bacteria from the soil. After the soil settles, take the liquid from the clarified part and spread it on a plate. The medium is a PDA plate solid medium, and put the plate into a constant temperature incubator. Cultivate in medium for 48 hours, after the colony grows, scrape out the place where there are colonies with an inoculation needle, put it into the PDB medium shaker and cultivate for 48 hours to obtain a bacterial suspension. The culture conditions are: inoculum size 1-2%, shaker speed 100-150rpm, 30-35°C. Potassium salt solution is 2% KCl solution. The preparation method is as follows: (1) after 48 hours of microbial culture, obtain th...

Embodiment 5

[0015] Implement by embodiment 1-2, each of above embodiment needs 48 Agarwood fragrant trees, produce agarwood after half a year.

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Abstract

The invention relates to an artificial Chinese eaglewood forming agent which consists of microorganisms and kali salt solution according to a volume ratio of 1:2 to 1:32. The microorganisms are selected from the following materials:(1) wood flour of a wound surface of a wounded aquilaria sinensis trunk; (2) ground powder on which Chinese eaglewood is formed; (3) dust particles around aquilaria sinensis trees, which has radius of 0.5 to 1 m; and (4) soil around the aquilaria sinensis trees, which has radius of 0.5 to 1 m. The kali salt solution is KCl solution with concentration of 2 percent. The microorganisms have the following culture conditions: the inoculation amount is 1 to 2 percent; a swing bed has rotating speed of 100 to 150 rpm; the temperature is 30 to 35 DEG C; a potato dextrose bouillon culture medium with pH of 7.0 is adopted. A method for preparing the artificial Chinese eaglewood forming agent comprises the following steps : (1) after culturing the microorganisms which are from different sources for 48h, respectively obtaining bacterium suspension; and (2) mixing the obtained microorganism bacterium suspensions and the kali salt solution according to proportion to obtain the artificial Chinese eaglewood forming agent. According to the invention, the defense reaction of aquilaria sinensis is utilized, Chinese eaglewood forming rate of Chinese eaglewood is improved and Chinese eaglewood forming time is shortened.

Description

Technical field: [0001] The invention relates to the field of an agarwood artificial aromatizing agent, in particular to the field of an artificial aromatizing agent combining microorganisms with potassium salts. Background technique: [0002] Agarwood is the only plant source of agarwood, a precious aromatic medicinal material, in my country. Agarwood itself does not form agarwood, but only produces a defensive reaction when it is attacked by the outside world, secretes resin, and forms agarwood after a long period of accumulation. At present, the main method of artificial incense is to use natural fungi to invade tree trunks; the second is to drill holes with iron wires, burn them, and bake them with fire to make the resin ooze out. [0003] However, the existing artificial fragrance-making method has the characteristics of low fragrance-making rate of agarwood and long time of fragrance-forming. [0004] Invention content: [0005] The purpose of the patent o...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N59/08A01P21/00A01G7/06
Inventor 叶汝章叶亚东张娟滕凌全利朱文华潘鹂胡娟霍世元李永忠韦珊路易斯伊格纳罗
Owner CHANGZHOU ADAM BIOTECH
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