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Antibacterial biological activity stent and preparation method thereof

A biologically active and antibacterial biological technology, applied in pharmaceutical formulations, medical preparations containing active ingredients, medical science, etc., can solve the problems of single function, antibacterial and biological activity, etc., to improve mechanical properties and promote vascular effect

Active Publication Date: 2015-01-07
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing scaffold materials have a single function, and often cannot take into account both antibacterial and biological activities.

Method used

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  • Antibacterial biological activity stent and preparation method thereof

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preparation example Construction

[0026] On the other hand, the present invention also provides a method for preparing the above-mentioned antibacterial bioactive scaffold, comprising the following steps:

[0027] The step of preparing gelatin microspheres is to prepare solid gelatin microspheres;

[0028] The step of preparing porous gelatin microspheres is to make the solid gelatin microspheres into porous gelatin microspheres;

[0029] In the active factor loading step, the active factor aqueous solution with a concentration of 0.01-150ug / ul is added dropwise to the porous gelatin microspheres. The amount of the active factor aqueous solution is preferably added dropwise to 20ul of the active factor aqueous solution per 2mg of porous gelatin microspheres, and the dropwise After the addition is completed, let it stand at room temperature, so that the active factor solution is fully impregnated on the dry microspheres, and the gelatin microspheres loaded with the active factor are obtained;

[0030] In the s...

Embodiment 1

[0039] 1. Preparation of gelatin microspheres

[0040] Take two parts of gelatin, add eight parts of distilled water to prepare gelatin with a concentration of 20% by mass, dissolve it in a constant temperature water bath at 42°C for 1 hour, and take 300mL soybean oil in a water bath at 42°C, and then Drop the gelatin solution into the oil at rpm, stir for 10 minutes to form a water / oil emulsion, then gradually lower the temperature of the mixed emulsion from 42°C to 0°C, keep the temperature for 15 minutes every time the temperature drops by 10°C, then add 200mL of acetone at 4°C, Adjust to 600rpm, continue stirring for 1h, wash with acetone and collect by centrifugation at 3200rpm, and finally soak in ethylene oxide for 2 days, change the solvent three times a day, wash with deionized water, freeze-dry for 2 days, and finally screen the particles with a sieve Gelatin microspheres with a diameter of 80-400 μm.

[0041] 2. Preparation of porous gelatin microspheres

[0042] ...

Embodiment 2

[0052] 1. Preparation of gelatin microspheres

[0053] Take four parts of gelatin, add six parts of distilled water to prepare a gelatin solution with a mass percentage of 40%, dissolve it in a constant temperature water bath at 50°C for 1 hour, and at the same time take 300mL of silicone oil in a water bath at 50°C, and then at 450 rpm Drop the gelatin solution into the oil at a rotating speed, stir for 10 minutes to form a water / oil emulsion, then gradually cool the mixed emulsion from 50°C to 0°C, keep the temperature for 15 minutes every time the temperature drops by 10°C, then add 200mL of acetone at 4°C, adjust the speed To 600rpm, continue to stir for 1h, wash with acetone and collect by centrifugation at 3200rpm, and finally soak in ethylene oxide for 2 days, change the solvent three times a day; wash with deionized water, then freeze-dry for 2 days, and finally use a sieve to screen the particle size 80-400μm gelatin microspheres.

[0054] 2. Loading with vascular en...

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Abstract

The invention relates to an antibacterial biological activity stent and a preparation method thereof. The method comprises the steps that gelatin microspheres are prepared; porous gelatin microspheres are prepared; gelatin microspheres carried with active factors are obtained; an antibacterial stent is prepared, wherein the preparation of the antibacterial stent comprises the steps that at least one of collagen, chitosan and silk fibroin and nano-silver grains are added to acetic acid or malonic acid to prepare a mixed solution, the mixed solution is poured into a die which is horizontally paved with the solid gelatin microspheres, the die is rapidly placed in an ultra cold refrigerator for freezing treatment, and the die is frozen and dried to obtain the antibacterial stent; and the antibacterial biological activity stent is prepared by the steps that the gelatin microspheres carried with the active factors are placed into normal saline to prepare a suspension liquid, and the suspension liquid is poured into the antibacterial stent to obtain the antibacterial biological activity stent after dried at the room temperature. According to the antibacterial biological activity stent and the preparation method thereof, pure natural polymers are taken as raw materials, the nano-silver grains and the bioactive factors are compounded to prepare the stent, so that the stent has the biological activity and antibacterial capacity, can effectively promote vascularization of cambium and can be used for skin injury healing.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to an antibacterial bioactive scaffold and a preparation method thereof. Background technique [0002] In daily life and work, human skin is damaged from time to time. At present, the repair of skin mainly relies on the migration and proliferation of vascular endothelial cells and fibroblasts and the formation of connective tissue to repair the dermis. This process often requires the assistance of skin grafting or artificial skin to complete. Although skin grafting can heal wounds, it leaves new wounds at the skin harvesting site, often leading to scar hyperplasia, and even because the skin harvesting is too deep, the skin donor site is difficult to heal itself, forming blisters, repeated ulcers and other unfavorable factors. [0003] Collagen is the main component of tissues such as skin, ligaments, tendons and other connective tissues, as well as the main component o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/18A61L27/24A61L27/22A61L27/20A61L27/04A61L27/60A61L27/54
Inventor 李小丽佘振定谭荣伟王明波刘伟强
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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