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Method for breeding novel variety of high-anthocyanin allium cepa

A technology of anthocyanin content and onion, applied in the direction of botany equipment and methods, application, plant genetic improvement, etc.

Inactive Publication Date: 2013-04-03
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there are no pure extracted anthocyanins in the market, at present, the anthocyanins needed by the human body are mainly taken in through food supplements

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] b. Preparation of anthocyanins in the sample to be tested: mash the internal scales of the onion, extract with a mixture of acetone and water (acetone:=water 7:3, volume ratio) 4 times the volume, and distill under reduced pressure below 40°C The organic solvent was removed, the water phase was washed with diethyl ether, and the volume was constant to obtain the anthocyanin solution of the sample to be tested, which was stored in a dark environment at 5°C for future use.

[0036] c. Sample determination: Wrap the test tube tightly with tin foil, leaving only the mouth of the tube for sample addition. Add 0.5ml of sample (the anthocyanin standard solution prepared in step a, or the anthocyanin solution of the sample to be tested prepared in step b) into the tube, and add 3.0ml of methanol solution containing 4% (volume fraction) vanillin Mix, then add 1.5ml of concentrated hydrochloric acid, mix thoroughly, develop color at room temperature for 15min, and measure color a...

Embodiment 1

[0046] Embodiment 1, the purpose onion of breeding high anthocyanin content

[0047] Set up an isolated area in the experimental field (spatial isolation of 2000 meters), the male sterile line of onion (Alliumcepa) B222A CGMCC No.6801 as the female parent and the inbred line (restorer line) of onion (Allium cepa) 2000-7 as the male parent -3-10 CGMCC No.6802 was planted in rows at a ratio of 4:1, and the plant and row spacing of the female parent and male parent were 25cm and 60cm, respectively. Female and male parents are pollinated by natural crosses. Seeds of the hybrid generation (F1 generation) were harvested from the female parent.

[0048]In the second year, 2400 F1 generation hybrid seeds (harvested from 20 female parents, 120 seeds per plant), 2400 male sterile onion (Allium cepa) B222A CGMCC No.6801 (female parent) Seeds, 2400 seeds of the inbred line (restorer line) onion (Allium cepa) 2000-7-3-10CGMCC No.6802 (male parent), and 2400 seeds of the existing purple o...

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PUM

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Abstract

The invention discloses a method for breeding a novel variety of high-anthocyanin allium cepa. The method for breeding hybrid allium cepa comprises the steps as follows: allium cepa B222A CGMCC No.6801 serves as a female parent; allium cepa 2000-7-3-10 CGMCC N0.6802 serves as a male parent; and the female parent and the male parent are hybridized to obtain the hybrid allium cepa from a generation F1. The obtained hybrid allium cepa has at least one of the following characteristics: the anthocyanin content of the bulb and / or output; and / or the weight of a single ball; and / or the disease resistance are higher than those of an allium cepa variety Red Top 1 and at least one of the allium cepa B222A CGMCC No.6801 and the allium cepa 2000-7-3-10 CGMCC N0.6802. Experiments show that the average content of the anthocyanin in the bulb of the F1-generation hybrid allium cepa bred and obtained through the method is considerably higher than that of the female parent, the male parent or the compared purple-skin allium cepa variety 'Red Top 1', and the average output of the hybrid allium cepa is also increased by 12% compared with that of the 'Red Top 1'.

Description

technical field [0001] The invention belongs to the field of plant breeding, and relates to a method for cultivating hybrid onions, in particular to a method for cultivating new varieties of high anthocyanin onions. Background technique [0002] Anthocyanins in plants are the most effective antioxidant substances in nature. It can supplement nutrition and eliminate harmful free radicals in the body. Its antioxidant properties are fifty times higher than vitamin E and twenty times higher than vitamin C. Anthocyanins in nature are widely found in fruits and vegetables, such as grapes, blueberries, black currants, cherries, red oranges, cranberries, strawberries, mulberries, basil, purple sweet potatoes, purple corn, tomatoes, red cabbage, purple carrots In the tissues of plants such as radish, purple radish, and purple onion. Eating products rich in anthocyanins is a simple and effective way to prevent and treat diseases such as cardiovascular and cerebrovascular diseases, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 梁毅王鼎慧张洪伟
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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