Inducing method for gynogenetic diploid of Nibea albiflora

Abstract

The invention provides an inducing method for a gynogenetic diploid of a Nibea albiflora, comprising the following steps of: (1) carrying out genetic inactivation on Nibea albiflora or Oplegnathus fasciatus sperms; (2) inseminating unfertilized ovums of the Nibea albiflora and inactivated sperms; (3) determining the starting time of chromosome reduplication of gynogenetic roes; and (4) screening the cold shock temperature and the duration of chromosome reduplication. The invention firstly provides a method for inducing Nibea albiflora ovum gynogenesis by using the sperms of the Nibea albiflora and the sperms of the Oplegnathus fasciatus to obtain a gynogenetic diploid fish fry, and the proper cold shock starting time, the cold shock temperature and the duration for Nibea albiflora ovum gynogenesis are screened out. The inducing method has the characteristics of convenience in operation, high efficiency, practicability and reliability; and a Nibea albiflora progeny obtained through inducing by using the method only contains the genetic materials of a female parent, so that the purification of an excellent gene can be accelerated, the excellent hereditary character can be rapidly fixed, and the inducing method has an important application value and a favorable popularization and application prospect on the aspect of breeding and sex control research of the Nibea albiflora.

Description

technical field [0001] The invention belongs to the field of cell engineering cultivation, and relates to a method for gynogenesis of seawater fish, in particular to a method for inducing diploid gynogenesis of yellow croaker. Background technique [0002] The use of cell engineering technology to cultivate fine aquatic species has important theoretical significance and broad application prospects. As a cell engineering technique, gynogenesis is an important way to improve fish species. The main purpose of using gynogenesis in fish farming is to establish pure lines and produce whole females. Through the hybridization technology between different pure lines, some recessive genes are almost completely removed, and new varieties (lines) with excellent traits are screened and bred from the hybrid offspring. Artificially induced gynogenesis has been widely used in the research and practice of various fish genetic breeding at home and abroad. For example: Japan uses this techn...

AI Technical Summary

Problems solved by technology

Therefore, it is necessary to carry out the research on artificial gynogenesis technology that can accelerate the breeding process, but there is no report on the artificial gynogenesis of yellow croaker at home and abroad.

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