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Polypeptide combined with FAP (Fibroblast Activation Protein)

A kind of specific and amino acid technology, applied in the field of peptides, can solve problems such as no breakthrough progress

Inactive Publication Date: 2013-03-20
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the "heterogeneity" of various tumors, the research on tumor-targeting molecules applicable to various pathological types has not yet achieved breakthroughs

Method used

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  • Polypeptide combined with FAP (Fibroblast Activation Protein)
  • Polypeptide combined with FAP (Fibroblast Activation Protein)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment F

[0027] Screening and Identification of Example FAP-specific Polypeptides

[0028] 1. Materials

[0029] Phage Cyclic Heptapeptide Library (Ph.D.-C7C TM Phage display peptide library): purchased from NEB company, 2×10 13 pfu / mL, diversity 2.7×10 9 , insert foreign sequences between the Kpn-I and Eag-I sites of the M13 phage cpIII protein, and the cysteines at both ends of the random heptapeptide form a disulfide bond, so that the presented random heptapeptide forms a relatively stable ring structure ; HRP-labeled mouse anti-M13 phage antibody: product of Abcam; FAP: product of sigma; other reagents were purchased from commercial libraries.

[0030] 2. Methods and Results

[0031] Resuscitation and cultivation of the recipient bacterium E.coli ER2738: Pick an inoculation loop from the glycerol frozen stock of E.coli ER2738 by aseptic technique, spread it on the LB-Tet plate, and culture it upside down at 37°C overnight. Pick a single colony, place it in 3ml of LB culture m...

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Abstract

The invention discloses a polypeptide combined with an FAP (Fibroblast Activation Protein). The polypeptide is obtained by screening from a phage display peptide library, can be specifically combined with the FAP, has high activity and has a sequence of SCDYNHHWC. The polypeptide can be used for preparing a broad spectrum probe aiming at a tumor matrix, and can meet the application requirement on the aspects of molecular imaging of tumors, targeting therapy of tumors, and the like.

Description

technical field [0001] The present invention relates to a polypeptide, in particular to a polypeptide binding to FAP. Background technique [0002] At present, malignant tumors have gradually replaced cardiovascular and cerebrovascular diseases as the number one killer of human beings. Recent statistics show that about 20 million cancer patients are newly discovered in my country every year, and nearly 1.5 million people die of cancer. Cancer deaths account for 1 / 5 of all deaths. Early diagnosis and treatment of tumors is the key to improving the cure rate and quality of life of patients, and how to find and use effective clinical methods to realize early diagnosis of tumors has become a hot field of basic research. [0003] More and more attention has been paid to the application research of molecular imaging in the diagnosis and treatment of tumors. The development of new high-affinity and specific molecular probes is an urgent problem in tumor molecular imaging. In th...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/14A61K51/08A61K47/42
Inventor 刘敏
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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