Method for automatically scanning and recording cell culture dish and tracking and positioning interested cell

Abstract

The invention relates to a method for automatically scanning and recording a cell culture dish and tracking and positioning an interested cell. The method is characterized by executing regionalization scanning, interested point recording in the scanning process and interested point tracking and positioning after the scanning is completed through a computer on the basis of a cell injection platform system. The method disclosed by the invention can be used for accurately acquiring the position of any point of the cell culture dish through the auxiliary design of the computer by selecting one point of the cell culture dish as a reference point, accurately positioning the interested point and automatically scanning the integral cell culture dish frame by frame, thereby achieving the seamless splicing of images; and the method disclosed by the invention can be used for overcoming the traditional defects of complex labor, irregularity and no memory function, greatly simplifying the scanning, querying and positioning operation on an injected cell, providing a more convenient and fast operational method for a microinjection technology, deepening the automatic operation degree and further increasing the work efficiency.

Description

technical field [0001] The invention relates to the field of automatic microinjection, in particular to a method for automatic scanning and recording of cell culture dishes and tracking and positioning of cells of interest. Background technique [0002] Microinjection (microinjection) technology is a microinjection technology operated under a microscope. A part of the cell (such as the nucleus, cytoplasm or organelles) or foreign substances (such as foreign genes, DNA fragments, messenger ribonucleic acid, proteins, drugs, etc.) can be injected into the cytoplasm or nucleus through a fine needle drawn from a glass capillary Inside. Microinjection technology is an important technology for studying the effects of various biomolecules, making transgenic animals, and cloning animals. Microinjection is used in a wide range of applications, from assisted (in vitro) cell fertilization techniques to the delivery of molecular and cellular building blocks. [0003] The traditional ...

AI Technical Summary

Problems solved by technology

[0005] Although the mechanized microinjection system is an important breakthrough in microinjection technology, there are also some important defects: the search for injected cells is still done manually, and the experimenter observes the cell culture dish by controlling the movement of the platform on the computer. , select the cells of interest to inject, after the injection, as long as the cell culture dish is slightly shifted or the cell culture dish is removed and installed on the stage again, it is difficult to find the injected cells, and, through manual search takes a lot of time

Therefore, the mechanized microinjection system is far from the real automatic microinjection technology.

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