Application of coreopsis tinctoria active part in preparation of anti-ageing cosmetic
An active part, anti-aging technology, applied in the field of plant extracts and daily chemicals, can solve the problems of literature and patent reports that have not found the anti-aging effect of snow chrysanthemum
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Embodiment 1
[0019] Snow chrysanthemum dry powder 20g, at room temperature (10-30°C) cold-soaked in 200ml of 30% ethanol, ultrasonically extracted twice, 10min each time (500W), combined the filtrates, recovered the solvent under reduced pressure to dryness, and extracted An appropriate amount of the substance was prepared into a 0.5mg / ml solution for later use, and its ability to scavenge DPPH free radicals was measured, so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0020] Result: IC of the scavenging DPPH free radical ability test of the crude extract of Snow Chrysanthemum 50 It was 428.3 μg / mL.
Embodiment 2
[0022] Snow chrysanthemum dry powder 20g, at room temperature with 60% ethanol 200ml cold soaking, ultrasonic extraction 2 times, each time 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure to dryness, take an appropriate amount of extract, and prepare 0.5 The mg / ml solution is ready for use, and its ability to scavenge DPPH free radicals is measured, so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0023] Result: IC of the scavenging DPPH free radical ability test of the crude extract of Snow Chrysanthemum 50 It was 120.8 μg / mL.
Embodiment 3
[0025] Snow chrysanthemum dry powder 20g, at room temperature with 70% ethanol 200ml cold soaking, ultrasonic extraction 2 times, each time 30min (500W), take the filtrate and combine, recover the solvent under reduced pressure to dryness, take an appropriate amount of extract, and prepare 0.5 The mg / ml solution is ready for use, and its ability to scavenge DPPH free radicals is measured, so as to compare with the activity of the active part of the snow chrysanthemum prepared later.
[0026] Result: IC of the scavenging DPPH free radical ability test of the crude extract of Snow Chrysanthemum 50 It was 51.2 μg / mL.
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