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Application of melaleuca alternifolia oil and preparation thereof

A technology of Melaleuca alternifolia oil and external preparations, applied in skin care preparations, medical preparations containing active ingredients, dressing preparations, etc., can solve the problems of Melaleuca alternifolia oil that have not yet been seen, and achieve The effect of broad application prospects

Inactive Publication Date: 2013-03-06
LB COSMECEUTICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there is no relevant report on the application of Melaleuca alternifolia oil as a Malassezia inhibitor

Method used

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  • Application of melaleuca alternifolia oil and preparation thereof
  • Application of melaleuca alternifolia oil and preparation thereof
  • Application of melaleuca alternifolia oil and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Inhibition zone experiment of Melaleuca alternifolia oil (Tianjin Tianxiang Biochemical Technology Co., Ltd.) against Malassezia.

[0035] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0036] Medium: Malassezia solid medium: glucose 40g / L, agar 14g / L, peptone 10g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, sterilized at 115°C and high pressure for 20min; Malassezia Bacteria liquid medium: glucose 40g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, sterilized at 115°C for 20min under high pressure conditions.

[0037] Experimental method: Subculture the test bacteria on the Malassezia solid medium to ensure the purification and viability of the colony, pick a single clone into 50ml Malassezia liquid medium, culture at 30°C for 24h, and use a turbidimeter to (Biological Mérieux) to adjust it to the concentration of McFarland No. 4 turbidimetric tube. Dilute the bacterium solution with the medium to make the final bacteriu...

Embodiment 2

[0043] Determination of minimum inhibitory concentration (MIC value) of Melaleuca alternifolia oil against Malassezia

[0044] Experimental strain: Malassezia furfur (Malassezia furfur, ATCC44344);

[0045] Medium: Malassezia liquid medium: glucose 40g / L, dissolved in distilled water, adjusted to pH 6.8-7.0, sterilized under high pressure at 115°C for 20min.

[0046] Experimental method: After the test bacteria were subcultured on the Malassezia solid medium, to ensure the purification and viability of the colony, pick a single clone into 50ml Malassezia liquid medium, culture at 30°C for 24h, and use turbidimetric The instrument (Biological Mérieux Company) adjusted it to the concentration of McFarland No. 4 turbidimetric tube. Dilute Melaleuca alternifolia oil directly with Malassezia liquid culture medium to test concentrations, which are 1000, 500, 250, 125, 62.5, 31.25 μg / ml, respectively. Add 100 μL of bacterial solution and 100 μL of medicinal solution to the microwel...

Embodiment 3

[0054] Preparation of conditioner:

[0055]

[0056]

[0057] Preparation process: raise the temperature of phase A to 80-85°C, and stir evenly. Raise the temperature of Phase B to 80-85°C and stir evenly. Add phase A into phase B, homogenize for 3 minutes. Cool down and stir. 3. Cool down to below 40°C, add phase C, and stir evenly.

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PUM

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Abstract

The invention discloses an application of melaleuca alternifolia oil as a malassezia inhibitor, and in particular relates to an application in preparing drugs for treating skin diseases caused by malassezia. The invention further discloses an external preparation and daily cosmetics containing the melaleuca alternifolia oil.

Description

technical field [0001] The present invention relates to the application and preparation of Melaleuca alternifolia oil. Background technique [0002] Malassezia (Pityrosporum) is a resident flora on the skin of humans and warm-blooded animals, belonging to the genus Lipophilic Basidiomycetes. As early as the 1990s, three species of Malassezia had been found on the skin of warm-blooded animals: Malassezia furfur, Malassezia pachyderm, and Malassezia symaxis. Then in people's research, it was found that there are 7 kinds of Malassezia, that is, in addition to the above 3 kinds, there are Malassezia globosa, Malassezia obtuse, Malassezia restrictive and Malassezia slofi bacteria. Malassezia mainly grows in the stratum corneum of human epidermis. It is a lipophilic flora. It mainly uses triacylglycerol and free fatty acid on the skin surface as nutrition, and the main factor affecting the secretion of triacylglycerol and free fatty acid Is the strength of the sebaceous gland f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K8/92A61P17/00A61P17/06A61P17/08A61P31/10A61Q5/02A61Q5/12A61Q19/00A61Q19/10
Inventor 孙常磊朱丽平翟春涛赵雪梅李成亮
Owner LB COSMECEUTICAL TECH CO LTD
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