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Application of non-small cell lung cancer pleural effusion tumor marker

A non-small cell lung cancer, tumor marker technology, used in biological testing, material testing, fluorescence/phosphorescence, etc., can solve problems such as increase, achieve the effect of improving sensitivity, overcoming the difficulty of distinguishing cell morphology, and reducing the rate of missed diagnosis

Inactive Publication Date: 2013-02-27
潘世扬
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, serum tumor markers may not necessarily be used as tumor markers for pleural effusion, as reported in the literature (Song Zhijun, Wu Guangping. Significance of CA724 and CA199 in clinical diagnosis of lung cancer patients. Chinese Medicine Guidelines, 2011, 9 (19): 18-19), CA72 -4 is significantly elevated in the serum of lung cancer patients, however, the diagnostic value of CA72-4 in the pleural effusion of lung cancer patients is far less than that in serum
As a new tumor marker with strong tissue specificity, SP70 has not been reported whether it is expressed in pleural effusion caused by non-small cell lung cancer patients

Method used

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  • Application of non-small cell lung cancer pleural effusion tumor marker
  • Application of non-small cell lung cancer pleural effusion tumor marker
  • Application of non-small cell lung cancer pleural effusion tumor marker

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1. Materials and Reagents

[0035] 1.1 Materials

[0036] From July 2011 to February 2012 in the First Affiliated Hospital of Nanjing Medical University, 108 cases of lung cancer patients with pleural effusion and 122 cases of non-cancerous pleural effusion diagnosed by medical history, clinical manifestations, CT or pathology were collected. Among the patients with cancerous pleural effusion, There were 78 males and 30 females; the age ranged from 32 to 84 years, with a median age of 65.0 years. Among the patients with non-cancerous pleural effusion, there were 82 males and 40 females; the age ranged from 58 to 90 years, with a median age of 70.0 years. The disease composition of patients with pleural effusion is shown in Table 1. Among them, 81 cases were lung cancer patients with staging data, 9 cases (6 cases of lung adenocarcinoma, 3 cases of lung squamous cell carcinoma) in the early group (Ⅰ / Ⅱ stage), and 9 cases in the late group (Ⅲ / Ⅳ stage). 72 cases (60 case...

Embodiment 2

[0103] 1Cell lines and clinical samples

[0104] 1.1 Source of cell lines

[0105] Human lung adenocarcinoma cell line SPC-A1 and human embryonic lung cell line HFL-1 were purchased from the Cell Bank of the Chinese Academy of Sciences.

[0106] 1.2 Collection and processing of clinical samples

[0107] 1.2.1 Collection of samples

[0108] A total of 93 pleural effusion exfoliated cell smears of patients with lung cancer diagnosed by medical history, clinical manifestations, CT or pathology and pleural effusion exfoliated cell smears of patients with benign lung diseases were collected from July 2011 to February 2012 in the First Affiliated Hospital of Nanjing Medical University. Among the 122 patients with cancerous pleural effusion, there were 66 males and 27 females; the age ranged from 43 to 84 years, with a median age of 65.0 years. Among the patients with non-cancerous pleural effusion, there were 82 males and 40 females; the age ranged from 58 to 90 years, with a med...

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Abstract

The invention belongs to the field of biological sample detection and discloses an application of a non-small cell lung cancer pleural effusion tumor marker. SP70 is used as the non-small cell lung cancer pleural effusion tumor marker to be applied to preparation of non-small cell lung cancer pleural effusion and / or pleural effusion cast-off cell diagnostic reagents. A monoclonal antibody of the SP70 is applied to preparation of the non-small cell lung cancer pleural effusion and / or pleural effusion cast-off cell diagnostic reagents. A direct immunofluorescence detection kit of non-small cell lung cancer pleural effusion cast-off cells contains the fluorescently-labeled monoclonal antibody of the SP70, 5% of bovine serum albumin (BSA) and 1% of a phosphate buffer solution (PBS). According to a great quantity of experimental researches, SP70 antigens can serve as a sensitive and specific detection index of malignancy pleural effusion caused by non-small cell lung cancers, simultaneously, the SP70 antigens in the pleural effusion and the pleural effusion cast-off cells can be detected, and sensitivity and specificity of carcinogenic pleural effusion diagnosis can be greatly improved.

Description

technical field [0001] The invention belongs to the field of biological sample detection and relates to the application of non-small cell lung cancer pleural effusion tumor markers. Background technique [0002] Pleural effusion, also known as pleural effusion, is one of the common clinical symptoms and can be caused by many diseases. In my country, tuberculosis is the first cause of disease, and malignant tumor is the second cause of disease. Pleural effusion caused by different diseases has very different countermeasures and patient prognosis. Therefore, the differential diagnosis of benign and malignant pleural effusion plays a vital role. [0003] Malignant pleural effusion is a common and distressing complication in patients with advanced tumors. 46% to 64% of patients with pleural effusion are caused by malignant tumors, and there is an increasing trend recently. Almost all malignant tumors (except primary brain tumors) can cause pleural effusion. Among the malignan...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N21/64
Inventor 潘世扬王芳黄珮珺徐建杨瑞霞孙瑞红黄蕾
Owner 潘世扬
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