Field in-situ dynamic monitoring method of ectomycorrhizal hyphae in ecological environment investigation

Abstract

The invention relates to a ectotrophic mycorrhiza mycelium field in situ dynamic monitoring method in ecological environment reconnaissance. The monitoring method can completely monitor mycelium embedded on host plant root systems, and simultaneously in situ monitoring for a long time, and accordingly development rule of mycorrhiza and flora at different growing periods can be dynamically obtained. Therefore, technical support is provided for ectotrophic mycorrhiza and flora action mechanism study, quantitative study and application, and further important actual ecological significance for subsequent land restoration.

Description

Technical field [0001] The invention relates to an in-situ dynamic collection method of soil microorganisms, in particular to a field in-situ dynamic monitoring method of ectomycorrhizal hyphae in ecological environment investigation. Background technique [0002] Ectomycorrhizal fungus is a mycorrhizal complex formed by fungi and the roots of higher plants. It is a common symbiotic fungus in nature. It can form symbiotic bodies with more than 80% of terrestrial plants. The main trees with ectomycorrhizas in my country are oak, pine, willow, linden, maple, walnut and birch. A large number of research reports have proved that fungal hyphae extend into the cells of the root cortex to form a hyphae net (called Hastelloy net), and at the same time, it spreads on the root surface to form a hyphae sheath to replace root hairs. Its main function is to expand the water and nutrient absorption area of ​​the root system; it can also secrete a variety of biological enzymes to improve the a...

AI Technical Summary

Problems solved by technology

[0003] At present, the observation method of ectomycorrhizal hyphae in the field is relatively simple, mainly by collecting the thinner root system of the host plant and bringing it back to the laboratory for film production observation

This method has great damage to the root system of the host plant during the root collection process, and cannot completely obtain the mycelium infected on the plant root system, and the mycelium fracture phenomenon is serious.