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Seawater spirulina selenium-rich culture method

A cultivation method and technology of spirulina, which are applied in the field of selenium-enriched cultivation of seawater spirulina, can solve the problems of difficult continuous circulatory cultivation, poor enrichment effect, and difficulty in effective utilization of the culture, achieve significant enrichment effect, and simplify the cultivation formula. , the effect of reducing training costs

Inactive Publication Date: 2013-02-13
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

The enrichment of selenium in freshwater spirulina needs to add a higher concentration of inorganic selenium, and the enrichment effect of low selenium concentration is not good. Selenium poisoning, algae cells produce monomethyl selenium and dimethyl selenium gas and red elemental selenium (Se) precipitation on (sub) selenate redox substances. The former is released in the air, which is harmful to algae cells, Toxic to the environment and humans, the latter is an insoluble precipitate that settles on the bottom of the culture vessel and is difficult to use effectively
Therefore, high-concentration inorganic selenium cultivates spirulina, which easily leads to failure of cultivation, and the culture is difficult to continuously circulate and cultivate. If the failed culture is dumped into the environment, it will not only waste selenium raw materials and other culture medium fertilizers, but also selenium and other culture medium fertilizers in the culture. Other nutrient-rich ingredients will create potential environmental problems

Method used

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  • Seawater spirulina selenium-rich culture method
  • Seawater spirulina selenium-rich culture method

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Experimental program
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Effect test

Embodiment 1

[0019] The algal species is a seawater SPH strain of Spirulina Platensis domesticated by the microalgae group of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. Based on the improved artificial seawater culture medium of Nanhai No. Ⅱ, the improved artificial seawater culture medium of Nanhai No. Ⅱ contains sodium bicarbonate 8g / L, sodium chloride 30 g / L, sodium nitrate 0.1 g / L, seven Ferrous sulfate water 0.1 g / L, urea 0.05 g / L, calcium chloride 0.04 g / L, N:P:K compound fertilizer (purchased from Sinochem Group Corporation, Sinochem brand compound fertilizer, N:P:K= 15:15:15) 0.3 g / L, potassium sulfate 1 g / L, magnesium sulfate 1 g / L, and water as the balance, add 150 ml of the above-mentioned Nanhai Ⅱ modified artificial seawater culture medium in a 500 ml Erlenmeyer flask , and adding selenium mother liquor with different volume concentrations of 10 mg / ml (sodium selenite is the solute, water is the solvent), so that the final selenium concentrations...

Embodiment 2

[0021] The algal species is a seawater SPH strain of Spirulina Platensis domesticated by the microalgae group of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. The culture medium is based on the improved artificial seawater culture medium of Nanhai II, which contains sodium bicarbonate 8g / L, sodium chloride 30 g / L, sodium nitrate 0.1 g / L, Ferrous sulfate heptahydrate 0.1 g / L, urea 0.05 g / L, calcium chloride 0.04 g / L, N:P:K compound fertilizer (purchased from Sinochem Group Corporation, Sinochem brand compound fertilizer, N:P:K =15:15:15) 0.3 g / L, potassium sulfate 1 g / L, magnesium sulfate 1 g / L, and water as the balance, add 150 ml Nanhai Ⅱ improved artificial seawater culture medium to a 500 ml Erlenmeyer flask , and add different volume concentrations of 10 mg ml -1 Selenium mother liquor (sodium selenite as solute, water as solvent), so that the final selenium concentrations were 0, 40, 80, 100, 120, 160 mg L - , and each concentration gradient ...

Embodiment 3

[0027] The algal species is a seawater SPH strain of Spirulina Platensis domesticated by the microalgae group of the South China Sea Institute of Oceanology, Chinese Academy of Sciences. The culture medium is based on the improved artificial seawater culture medium of Nanhai No. Artificial seawater medium contains sodium bicarbonate 8 g / L, sodium chloride 30 g / L, sodium nitrate 0.1 g / L, ferrous sulfate heptahydrate 0.1 g / L, urea 0.05 g / L, calcium chloride 0.04 g / L L, N:P:K compound fertilizer (purchased from Sinochem Group Corporation, Sinochem brand compound fertilizer, N:P:K=15:15:15) 0.3 g / L, potassium sulfate 1 g / L, magnesium sulfate 1 g / L, the balance is water, and then 16L of Nanhai Ⅱ modified artificial seawater medium was added to a 20 L glass cylinder reactor, and then 10 mg ml -1 selenium stock solution (sodium selenite), so that the final concentration of selenium is 40 mg L - , so as to obtain the culture medium of seawater spirulina, and then add algae species, i...

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Abstract

The invention discloses a seawater spirulina selenium-rich culture method. The method comprises the following steps of: selecting one or combination of more of the seawater-naturalized spirulina platensis, spirulina maxima and microcystis aeruginos as the algae seed; based on the natural seawater, artificial seawater or the mixture of the two at any ratio, adding a selenium element with concentration of 1-160mg / L to serve as a culture medium; inoculating the algae seed into a photobioreactor containing the culture medium for culture; and then collecting the cultured spirulina body to obtain the seawater spirulina which is the selenium-rich seawater spirulina. Through the invention, the method is simple, the selenium addition concentration is low, the culture medium can be recycled for a long time, the operation is simple and convenient, the selenium enrichment ability is strong, the cost is low, the method is green and safe, etc. The bioactive substance of the seawater spirulina generates a synergistic effect with organic selenium in the algae body to realize the function, and can be applied to various diseases caused by selenium lack and oxidative damage of oxygen free radical for the selenium supplement additive, oxidation resistance, aging resistance, tumor resistance, Keshan disease prevention and treatment and the like.

Description

Technical field: [0001] The invention relates to a method for cultivating spirulina, in particular to a method for cultivating seawater spirulina enriched in selenium. Background technique: [0002] Selenium is one of the essential trace elements for human body and other mammals, and it has important physiological functions. It can improve the immunity of the body; it can significantly prevent and inhibit the occurrence, proliferation, migration and recurrence of tumors caused by spontaneous, transplantation and chemical carcinogens in animals; it also plays an important role in preventing cardiovascular diseases and anti-aging. Selenium supplementation can prevent and treat many diseases, such as Keshan disease, cardiovascular disease, liver disease and tumors, etc. The application of selenium to prevent tumors and other diseases is an emerging hotspot. Based on the high biological activity and low toxicity of organic selenium, the research on the enrichment of organic sel...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 吴华莲向文洲何慧何晓艳成华范洁伟
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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