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Triterpenoid saponin compound extracted from clematis tangutica and application thereof

A technology of triterpenoid saponins and Clematis clematis, which is applied in the field of medicine, can solve the problems of chemical structure and anti-myocardial ischemia activity that have not been reported before.

Inactive Publication Date: 2014-07-23
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We systematically separated the total saponins of Clematis clematis and obtained a new triterpenoid saponin compound, whose chemical structure and anti-myocardial ischemia activity have not been reported before

Method used

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  • Triterpenoid saponin compound extracted from clematis tangutica and application thereof
  • Triterpenoid saponin compound extracted from clematis tangutica and application thereof
  • Triterpenoid saponin compound extracted from clematis tangutica and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1: Extraction and separation of compounds

[0017] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into a coarse powder, take 3 kg, add 15 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 3 kg. Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 540 g of ethanol extract. The extract was dispersed in 4.5 liters of water, and extracted 3 times with petroleum ether, 4.5 liters each time. The extracted aqueous phase was then extracted 3 times with n-butanol, 4.5 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 120 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the elue...

Embodiment 2

[0022] Collect the whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province as a raw material. After the raw material is crushed into coarse powder, take 3 kg, add 12 liters of ethanol with a concentration of 70% and carry out reflux extraction. Extract 3 times, each time 2 Hour. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 508 g of ethanol extract. The extract was dispersed in 3 liters of water, and extracted 3 times with petroleum ether, 3 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 3 liters each time, the n-butanol extract was combined, and the solvent was recovered under reduced pressure to obtain 112 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the volume ratio was 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) and the gradient elution was chloroform-methanol-water mixed solvent, and 1...

Embodiment 3

[0024] The whole herb of Clematis clematis in Huangnan Autonomous Prefecture, Qinghai Province was collected as a raw material. After the raw material was crushed into a coarse powder, 1 kg was taken, and 3 liters of methanol were added for reflux extraction. A total of 3 extractions were performed for 1 hour each time. The extracts were combined, and the solvent was recovered under reduced pressure to obtain 150 g of methanol extract. The extract was dispersed in 0.6 liters of water, and extracted 3 times with petroleum ether, 0.6 liters each time. The extracted aqueous phase was extracted 3 times with n-butanol, 0.6 liters each time, the n-butanol extracts were combined, and the solvent was recovered under reduced pressure to obtain 45 grams of total saponin extract. The total saponins were subjected to silica gel column chromatography, and the chloroform-methanol-water mixed solvent with a volume ratio of 8:2:1 to 6.5:3.5:1 (the lower layer was taken as the eluent) was used...

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Abstract

The invention provides a triterpenoid saponin compound extracted from clematis tangutica. The molecular formula of the triterpenoid saponin compound is C59H96O27. The chemical structure of the compound is determined as 3beta-O-[3-O-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabinopyranoside]-21alpha-hydroxy-Chinese ivy-28-O-alpha-L-rhamnopyranosyl-(1->4)-beta-D-glucopyranose-(1->6)-beta-D-glucopyranose(21alpha-dydroxy-3beta-O-[3-O-alpha-L-rhamnopyranosyl-(1->2)-alpha-L-arabinopyran osyl]-hederagenin-28-O-alpha-L-rhamnopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranoside) by adopting spectrum technologies such as a high-resolution mass spectrum, a two-dimensional nuclear magnetic resonance spectrum and the like and a chemical method. As proved by an in-vitro anti-myocardial ischemia active result, the compound plays a remarkable role in protecting myocardial injury induced by an anoxic / reoxygenation model. The invention provides a lead compound for treating myocardial ischemia diseases, and the triterpenoid saponin compound can become a cardiovascular medicament.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to triterpene saponins extracted from Clematis clematis and its application, specifically a new triterpene saponin extracted and separated from the traditional Tibetan medicine Clematis clematis Compounds and their application in the preparation of cardiovascular drugs. Background technique [0002] Ischemic heart disease is the main cause of death in humans, and its high morbidity and mortality seriously affect the quality of life of patients and are life-threatening. At present, the drugs for clinical treatment of cardiovascular diseases mainly include nitrates, calcium antagonists and β receptor blockers. Although these drugs have achieved certain clinical curative effects, they all have different degrees of toxic and side effects and drug resistance. Therefore, the development of new efficient and safe cardiovascular drugs is an urgent need for the treatment of this disease. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P9/00
Inventor 张伟汤海峰文爱东奚苗苗纪兰菊武菲菲马宁
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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