Alcohol production process

A technology for ethanol and alcohols, which can be used in microorganisms, biochemical instruments, biofuels, etc., and can solve the problem of high energy consumption for alcohol recovery

Inactive Publication Date: 2013-01-02
新西兰郎泽科技公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at lower alcohol levels in the fermentation broth, the energy consumption of alcohol recovery is relatively higher

Method used

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  • Alcohol production process
  • Alcohol production process
  • Alcohol production process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] Example 1: Batch fermentation in CSTR

[0121] Solution A

[0122] NH 4 Ac 3.083g KCl 0.15g

[0123] MgCl 2 .6H 2 O 0.61g NaCl 0.12g

[0124] CaCl 2 .2H 2 O 0.294g distilled water up to 1L

[0125] Solution B

[0126]

[0127] Solution C

[0128]

[0129] Preparation of Cr(II) solution

[0130] A 1 L three-necked flask was fitted with gas-tight inlet and outlet to allow working under inert gas and subsequent transfer of the desired product to a suitable storage flask. The flask was filled with CrCl 3 .6H 2 0 (40g, 0.15mol), zinc particles [20 mesh] (18.3g, 0.28mol), mercury (13.55g, 1mL, 0.0676mol) and 500mL of distilled water. use N 2 After purging for 1 hour, the resulting mixture was heated to about 80° C. to start the reaction. at constant N 2 After stirring under flow for 2 hours, the mixture was cooled to room temperature and stirring was continued for 48 hours, at which point the reaction mixture became a dark blue...

Embodiment 2

[0139] The alcohol tolerance of Clostridium autoethanogenum DSM23693 was tested in serum bottles ( Figure 5 ). Ethanol was added at various concentrations to cultures actively growing at 37 °C in PETC medium (Table 1) with 30 psi steel plant gas (approximately 48% CO, 32% N 2 , 2%H 2 and 18%CO 2 ) as a substrate. By HPLC using an Agilent 1100 series HPLC system equipped with a RID (refractive index detector) operated at 35°C and an Alltech 10A-2000 organic acid column (150 x 6.5mm, particle size 5 μm) maintained at 60°C Analyze ethanol concentration. Use slightly acidified water (0.005MH 2 SO 4 ) as mobile phase, the flow rate is 0.7ml / min. To remove proteins and other cellular residues, 400 μl samples were mixed with 100 μl of 2% (w / v) 5-sulfosalicylic acid and centrifuged at 14,000 x g for 3 minutes to separate precipitated residues. Then 10 μl of the supernatant was injected into the HPLC for analysis.

[0140] At the lowest alcohol concentration (0.2 g / L, where n...

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Abstract

The invention relates to the microbial fermentation of gaseous substrates, particularly to methods of mitigating and / or reducing alcohol toxicity effects on a microbial culture at elevated alcohol concentrations during fermentation. The invention relates particularly to microbial fermentation of substrates comprising CO and the effects of alcohol toxicity are reduced or mitigated by maintaining the temperature below the optimum operating temperature by cooling the fermentation broth.

Description

technical field [0001] The present invention generally relates to methods for the production of products, particularly alcohols, by microbial fermentation. In particular, the present invention relates to methods for reducing the toxic effects of alcohols during the fermentation of CO-containing substrates. Background technique [0002] Ethanol is rapidly becoming the world's primary hydrogen-rich liquid transportation fuel. Worldwide ethanol consumption in 2005 was estimated at 12.2 billion gallons. The global market for the fuel ethanol industry has been expected to continue to grow rapidly in the future because of increased interest in ethanol in Europe, Japan, the United States, and some developing countries. [0003] For example, in the United States, ethanol is used to produce E10—a 10 percent blend of ethanol in gasoline. In ElO blends, the ethanol component acts as an oxygenating agent, improving the efficiency of combustion and reducing the production of air pollu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12M1/34C12M1/38C12R1/145
CPCY02E50/17C12P7/065Y02E50/10
Inventor B·D·黑斯特拉
Owner 新西兰郎泽科技公司
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