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Triggered cargo release from nanoparticle stabilized liposomes

A nanoparticle and gold nanoparticle technology, applied in liposome delivery, drug combination, drug delivery, etc., can solve the problems of PEGylated liposome with less local drug delivery and less bacterial infection

Inactive Publication Date: 2013-01-02
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, PEGylated liposomes are rarely used for topical drug delivery, especially for the treatment of bacterial infections

Method used

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  • Triggered cargo release from nanoparticle stabilized liposomes
  • Triggered cargo release from nanoparticle stabilized liposomes
  • Triggered cargo release from nanoparticle stabilized liposomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] The preparation of the gold nanoparticle of embodiment 1-carboxyl modification

[0096] Preparation of carboxy-modified gold nanoparticles (AuC): According to complete and detailed descriptions in other documents (Aryal, S. et al., Spectroscopic Identification of S-Au Interaction in Cysteine ​​Capped Gold Nanoparticles.Spectrochim.ActaA2006, 63, 160-163; Patil , V. et al., Role of Particle Size in Individual and Competitive Diffusion of Carboxylic Acid Derivatized Colloidal Gold Particles in Thermally Evaporated Fatty Amine Films. Langmuir 1999, 15, 8197-8206) in sodium borohydride reduction method to prepare AuC. Briefly, at ice-cold temperature with 0.005 g NaBH 4 reduced HAuCl 4 aqueous solution (10-4M, 50 mL), thus forming bare gold nanoparticles (AuB). By mixing AuB with MPA (mercaptopropionic acid, 4×10 -4 M) Overnight incubation to carboxyl functionalize AuB. The resulting AuCs were washed 3 times through an Amicon Ultra-4 centrifugal filter with a molecular ...

Embodiment 2

[0106] Example 2 - MRSA Treatment via Liposome Pore Formation

[0107] experimental method

[0108] Materials: Hydrogenated L-α-phosphatidylcholine (egg PC) and cholesterol were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Sephadex G-75 was purchased from Fisher Scientific (Pittsburgh, PA). 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt (ANTS) and p-xylene-bispyridinium bromide (DPX) were obtained from Invitrogen (Carlsbad, CA). Poly(ethylene glycol) methyl (Mn = 2000 Da) and Triptic Soy Broth (TSB) were purchased from Sigma Aldrich (St Louis, MO). tetrachloroauric acid (HAuCl 4 ) and sodium borohydride (NaBH 4 ) from ACROS Organics (Geel, Belgium). Chitosan-50 was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

[0109] Preparation and characterization of AuChi and AuChi-liposomes

[0110] Preparation of chitosan-modified gold nanoparticles (AuChi) by sodium borohydride reduction technique (Pornpattananangkul, D. et al., ACS Nano2...

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Abstract

A new approach to control the fusion activity of liposomes by adsorbing biocompatible nanoparticles to the outer surface of phospholipid liposomes is disclosed. The biocompatible nanoparticles effectively prevent liposomes from fusing with one another. Release of cargo from the liposome is accomplished via trigger mechamisms that include pH triggers, pore forming toxing triggers and photosentisitve triggers. Dermal drug delivery to treat a variety of skin diseases such as acne vulgaris and staph infections is comtemplated.

Description

[0001] Related applications [0002] This application claims priority to U.S. Provisional Application Serial No. 61 / 3 13,512, filed March 12, 2010, and U.S. Provisional Application Serial No. 61 / 439,141, filed February 3, 2011, both of which are Incorporated herein by reference in its entirety. [0003] Statement Regarding Federally Sponsored Research or Development [0004] This invention was made with government support under grants from the National Institutes of Health (grant numbers U54CA1 19335, R01AI067395-01, and 1R21AI088147-01A1), and the National Science Foundation (grant number CMMI-1031239). The government has certain rights in this invention. [0005] Incorporation by reference of information submitted electronically [0006] Not applicable technical field [0007] The present teachings relate to triggered cargo release of nanoparticle-liposome compositions and methods of use. Background of the invention [0008] The use of nanoparticles to differentially ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/26A01N25/28A61K9/127
CPCA61K9/0014A61K9/1271A61K9/127A61K9/06A61K9/5115A61K9/0019A61P17/00A61P31/00A61P31/04A61P35/00
Inventor 张良方迪萨亚·波恩帕坦纳南库尔俊铭·E·黄
Owner RGT UNIV OF CALIFORNIA
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