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Preparation method for promoting self generation of human bone marrow hematopoietic stem cells

A production method and bone marrow technology, applied in the fields of life science and biology, can solve the problem of small rejection of umbilical cord blood stem cells, and achieve the effect of partial improvement of original symptoms and partial recovery of functions

Active Publication Date: 2012-12-26
闫利琴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Disadvantages of the existing stem cell transplantation technology: Two types of adult stem cell transplantation can cause graft rejection due to improper matching; umbilical cord blood stem cells are much less likely to experience rejection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A preparation method for autologously producing bone marrow hematopoietic stem cells, comprising performing the following steps in sequence:

[0063] a. Collect bovine placenta and fetal bovine bone marrow at a ratio of 1:1 by weight, and store at low temperature in liquid nitrogen;

[0064] b After being washed with pure water, it is sterilized by conventional methods of ozone;

[0065] C adopts refrigerated centrifuge to carry out protein separation, fat separation, active substance separation with ultrafiltration, wherein, the rotating speed of centrifuge is 9100 revs / min; Stop after 0.5 hour; Obtain sediment like this,

[0066] d Add pepsin (with a content of more than 99%) at a ratio of 30 mg of pepsin per gram of sediment, and stay at 35°C for 100 minutes; adjust the pH value to 1.7;

[0067] e add water at a ratio of 6:1 by weight, extract for 4 hours; obtain fetal bovine bone marrow active polypeptide;

[0068] f Take the above fetal bovine bone marrow active ...

Embodiment 2

[0071] A preparation method for autologously producing bone marrow hematopoietic stem cells, comprising performing the following steps in sequence:

[0072] a. Collect bovine placenta and fetal bovine bone marrow in a ratio of 2:1 by weight, and store at low temperature in liquid nitrogen;

[0073] b After being washed with pure water, it is sterilized by conventional methods of ozone;

[0074] C adopts refrigerated centrifuge to carry out protein separation, fat separation, active substance separation with ultrafiltration, wherein, the rotating speed of centrifuge is 9000 rpm; Stop after 1 hour; Obtain deposit like this,

[0075] d Add pepsin (with a content of more than 99%) at a ratio of 30 mg of pepsin per gram of sediment, and stay at 40°C for 120 minutes; adjust the pH value to 1.7;

[0076] e add water at a ratio of 6:1 by weight, and extract for 5 hours; obtain fetal bovine bone marrow active polypeptide;

[0077] f Take the above fetal bovine bone marrow active poly...

Embodiment 3

[0080] A preparation method for autologously producing bone marrow hematopoietic stem cells, comprising performing the following steps in sequence:

[0081]a. Collect bovine placenta and fetal bovine bone marrow at a ratio of 1.5:1 by weight, and store at low temperature in liquid nitrogen;

[0082] b After being washed with pure water, it is sterilized by conventional methods of ozone;

[0083] c adopt refrigerated centrifuge to carry out protein separation, fat separation, active substance separation by ultrafiltration, wherein, the rotating speed of centrifuge is more than 9000 rev / min; Stop after 0.8 hour; Obtain sediment like this;

[0084] d Add pepsin (with a content of more than 99%) at a ratio of 30 mg of pepsin per gram of sediment, and stay at 38°C for 110 minutes; adjust the pH value to 1.7;

[0085] e. Add water at a weight ratio of 6:1, extract for 4-5 hours; obtain fetal bovine bone marrow active polypeptide;

[0086] f Take the above fetal bovine bone marrow ...

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Abstract

The invention provides a preparation method for promoting generation of human bone marrow hematopoietic stem cells, and relates to a manufacture method of stem cells preparation. The method successively comprises the following steps of: a, collecting bovine placenta and fetal bovine marrow; b, cleaning, and sterilizing; c, separating protein, fat and active materials through a freezing centrifugal machine in an ultrafiltration method, so that the sediment is obtained; d, adding pepsase; e, adding water, and extracting to obtain fetal bovine marrow active polypeptide; f, getting the fetal bovine marrow active polypeptide, banana seedcase element and echinacea polysaccharide to be uniformly mixed to obtain marrow polysaccharide; g, getting the marrow polysaccharide, adding the water, and adding strontium, zinc, selenium, iodine, metasilicic acid, calcium, magnesium, potassium, sodium, iron, copper, lithium and nickel, and adjusting a pH value to be alkalescent, and then compounding to obtain a biological activity oral liquid preparation suitable for a human body. The preparation method has the beneficial effect that the human medullary hematopoiesis can be promoted to produce the hematopoietic stem cells; and an unique effect of treating various diseases can be achieved; and the preparation method is safe, efficient and has low cost, and prevention, health care and treatment effects can be achieved.

Description

technical field [0001] The invention relates to the fields of life science and biotechnology, and further relates to a method for manufacturing stem cell preparations, especially a liquid preparation method for human autogenous bone marrow hematopoietic stem cells. Background technique [0002] Life science is one of the fastest-growing disciplines in the 20th century, and has become the most eye-catching field in natural science. The discovery and application of hematopoietic stem cell transplantation technology has brought new hope for human beings to overcome diseases. [0003] On May 14, 2012, the Ministry of Science and Technology issued the "Twelfth Five-Year Plan" of the National Major Scientific Research Program of Stem Cell Research with Guo Ke Fa Ji [2012] No. 627. [0004] On November 3, 2009, Premier Wen Jiabao made a speech at the 60th anniversary meeting of the Chinese Academy of Sciences and pointed out that stem cell research has promoted the development of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C08L5/00C08L89/00C08K5/16A61K38/01A61P7/00A61P43/00A61K36/88A61K35/50A61K35/28A61K33/00A61K33/04A61K33/06A61K33/18A61K33/26A61K33/30A61K33/34A61K31/715A61K35/54
Inventor 闫利琴
Owner 闫利琴
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