Method for increasing nitrogen utilization efficiency of rape
A technology of rapeseed nitrogen and efficiency, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problem of combined research, there are few reports on the activity of tonoplast membrane proton pump and nitrogen utilization efficiency, there is no universal, effective methods, etc., to achieve the effect of reducing the content, improving the efficiency of nitrogen use, and efficient use of nitrogen
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[0014] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the following examples are used to describe the present invention in detail.
[0015] Cultivation of rapeseed seedlings: Soak the seeds of rapeseed varieties X-2 and X-6 in 20% (v / v) sodium hypochlorite for surface disinfection for 30 minutes, rinse with distilled water several times, and place them in a glass mesh room on September 30. For germination in soil culture, the composition of Hoagland complete nutrient solution used is 5 mmol / L KNO 3 , 1 mmol / L KH 2 PO 4 , 7 mmol / L MgSO 4 , 5 mmol / L Ca(NO 3 ) 2 4H 2 O, 3 mmol / L Fe-EDTA, 0.5 mg / L B, 0.5 mg / L Mn, 0.05 mg / L Zn, 0.02 mg / L Cu, 0.01 mg / L Mo, pour the nutrient solution every 5 days, 100 ml. The average day and night temperature in the glass mesh room is 29°C / 19°C, the natural humidity and light, and the light intensity is 205-230 Ue / (m 2 .s).
[0016] Transplanting of rapeseed se...
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