Method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion

A technology of Emilia Radix and full time period is applied in the field of simultaneous determination of the content of three components by three-wavelength fusion in the whole period of traditional Chinese medicine Emmanuel Radix. Ensure the standardization of the process and the effect of simple operation

Active Publication Date: 2012-12-12
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

At present, the quality of commercially available emblica medicinal materials is uneven, so the stability of emblica medicinal materials is difficult to guarantee

Method used

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  • Method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion
  • Method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion
  • Method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion

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Embodiment Construction

[0030] The method for simultaneously determining the content of three components by three-wavelength fusion of the traditional Chinese medicine Emlical emblica in a full period of time comprises the following steps:

[0031] 1. Preparation of the test solution:

[0032] Take 3 g of emblica medicinal material (passed through a 16-mesh sieve), accurately weigh it, put it in a round-bottomed flask, add 33 mL of 70% ethanol accurately, heat to reflux for 1.5 hours, extract three times, recover the combined filtrate, add water to make the solution final The concentration is 0.15g / ml, and 17mL of the filtrate is accurately absorbed, added to the polyamide resin column, washed with 90mL of distilled water, and then eluted with 105mL of 50% ethanol. The ethanol eluate is collected, evaporated to dryness, and the residue is dissolved by adding methanol. Transfer to a 10mL measuring bottle, add methanol to make up to the mark, shake well, filter, and take the filtrate to obtain the fina...

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Abstract

The invention discloses a method for simultaneously determining content of three components for traditional Chinese medicine phyllanthus emblica through whole-time three-wavelength fusion. According to the method, a modern analysis detection method and an information processing method are combined, spectrogram information of marker components of Chinese herbal medicines is effectively fused, determined components are the largest ultraviolet absorption wavelengths, the signal to noise ratio of the determined components is increased, the defect of insufficient information amount of single wavelength and index detection can be overcome, and internal quality of Chinese herbal medicines can be reflected perfectly. The quality control method of the three components is simple in operation, stable and reliable, good in reproducibility, and capable of being applied to quality evaluation and control of Chinese herbal medicines and new drug development, particularly serving as one of indexes of quality control and identification for the traditional Chinese medicine phyllanthus emblica.

Description

field of invention [0001] The invention belongs to the technical field of detecting the quality of Chinese medicinal materials by means of modern analysis and detection means and information processing means, and relates to a method for controlling the content of multi-index components of Chinese medicinal materials, more specifically, a full-time three-wavelength method for the traditional Chinese medicine Amla emblica A method for simultaneously determining the contents of three components combined. Background technique [0002] According to the first part of the Pharmacopoeia of the People's Republic of China in 2010, this product is the dried mature fruit of Phyllanthus emblica L., which is harvested from winter to spring when the fruit is ripe, removed from impurities, and dried. Phyllanthus emblica was first recorded in "Southern Vegetables and Trees". Phyllanthus emblica taste is sweet, cold, non-toxic, used for clearing away heat and swelling, relieving throat and m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 孟宪生张云坤王飞包永睿王帅
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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