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Lactate dehydrogenase A acetylation activator and application thereof

A lactate dehydrogenase and acetylation technology, which is applied in the fields of molecular biology and medicine to achieve simple and low-cost effects of tumor metabolism diagnosis and treatment

Inactive Publication Date: 2012-12-05
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression of LDHA in all tumors has always been a hotspot and difficulty for biologists, especially the post-translational modification of LDHA and its protein stability have not been reported internationally

Method used

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  • Lactate dehydrogenase A acetylation activator and application thereof
  • Lactate dehydrogenase A acetylation activator and application thereof
  • Lactate dehydrogenase A acetylation activator and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Immunohistochemical detection

[0041] 1) Experimental materials

[0042] Paraffin sections of pancreatic tissue were obtained from the Department of General Surgery, Shanghai Sixth People's Hospital; LDHA acetylated antibody was prepared by itself; LDH was purchased from the company.

[0043] 2) Sample detection:

[0044] A total of 71 cases of pancreatic cancer and 38 adjacent tissues were detected in the experiment, and 3 cases of paraffin sections were collected for each case, and HE staining, immunohistochemical analysis of acetylation of LDH and LDHAK5 were performed, and different visual fields were selected to measure the intensity of staining with software. Finally, paired t-test was performed.

[0045] 3) Test results:

[0046] Such as Figure 4 As shown, the test results showed that the acetylation level of LDHA K5 in tumor tissue was significantly lower than that in adjacent normal tissue.

[0047] Experimental results show that the acetylation level of...

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PUM

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Abstract

The invention belongs to the field of molecular biology and medicine, and relates to a lactate dehydrogenase A acetylation activator and an application thereof. The lactate dehydrogenase A acetylation activator comprises an LDHA K5 acetylation activator which can be used for effectively activating acetylation of K5. The invention further provides a corresponding antibody and a method for detecting LDHA K5 acetylation. The detection method can be used for detecting the level of LDHA K5 acetylation in a tumor sample, is simple, practicable, rapid and effective, and is low in cost; and the LDHA K5 acetylation is detected by using the method, and inhibition is performed specific to the level of LDHA K5 acetylation, so that a novel simple and convenient way is provided for tumor metabolism diagnosis and treatment.

Description

technical field [0001] The invention belongs to the fields of molecular biology and medicine, and relates to a lactate dehydrogenase A (LDHA) acetylation activator and its application, and also relates to an antibody and a detection method for detecting LDHAK5 acetylation. Background technique [0002] Tumors are metabolic diseases derived from the concept of tumor cell metabolism proposed by Otto Warburg in the 1930s, that is, the metabolic regulation of cells in rapidly growing tissues (such as embryos or tumors) is different from that of normal mature cells through glycolysis or the Warburg effect , cancer cells absorb glucose more efficiently than other cells and produce energy and grow rapidly (Warburg, 1956). Studies have shown that normal cells only carry out glycolysis under the condition of hypoxia, while tumor cells preferentially carry out glycolysis even without hypoxia, consume more glucose and produce more lactic acid, which is the famous Warburg effect, for w...

Claims

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Application Information

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IPC IPC(8): A61K38/45A61K38/44C07K16/40G01N33/574A61P35/00
Inventor 雷群英赵地邹邵武周鑫管坤良熊跃刘颖董波
Owner FUDAN UNIV
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