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SiRNA (small interfering ribonucleic acid) inhibiting PDCD4 (programmed cell death4) gene expression and application of siRNA

A gene expression, gene technology, applied in the field of molecular biology, can solve problems such as less function

Inactive Publication Date: 2012-10-10
JINAN UNIVERSITY
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Problems solved by technology

[0004] However, there are few functional studies of PDCD4 in leukemia. Recent studies have found that in acute myelogenous leukemia (Acute myelogenous leukemia, AML), the expression of PDCD4 can promote retinoic acid-induced granulocyte differentiation (Ozpolat B, Akar U, Steiner M, et al. Mol Cancer Res, 2007, 5:95.)

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  • SiRNA (small interfering ribonucleic acid) inhibiting PDCD4 (programmed cell death4) gene expression and application of siRNA
  • SiRNA (small interfering ribonucleic acid) inhibiting PDCD4 (programmed cell death4) gene expression and application of siRNA
  • SiRNA (small interfering ribonucleic acid) inhibiting PDCD4 (programmed cell death4) gene expression and application of siRNA

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Embodiment 1

[0028] (1) Design and synthesis of PDCD4 siRNA

[0029] According to the known sequence of PDCD4 in the GenBank gene bank (Gene ID: 27250, NM_145341, NM_014456), according to the siRNA design principle, combined with the online design tool siCatch provided by Ambion TM siRNA Design designed the following three sequences for different siRNA target positions in the coding region:

[0030] PDCD4 siRNA sequence one:

[0031] Sense strand: 5'-CACCAAUCAUACAGGAAUA-3',

[0032] Antisense strand: 5'-UAUUCCUGUAUGAUUGGUG-3';

[0033] PDCD4 siRNA sequence two:

[0034] Sense strand: 5'-GGAGCGGUUUGUAGAAGAA-3',

[0035] Antisense strand: 5'-UUCUUCUACAAACCGCUCC-3';

[0036] PDCD4 siRNA sequence three:

[0037] Sense strand: 5'-GGUGGAAAGCGUAAAGAUA-3',

[0038] Antisense strand: 5'-UAUCUUUACGCUUUCCACC-3';

[0039] The 3' end of each of the above siRNA sequences is hung with dTdT;

[0040] The siRNA general control was used as a random control (the siRNA general control has at least 5 b...

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Abstract

The invention discloses a siRNA (small interfering ribonucleic acid) inhibiting PDCD4 (programmed cell death4) gene expression and application of the siRNA. The sense strand sequence is: 5'-GGAGCGGUUUGUAGAAGAA-3', and the anti-sense strand sequence is: 5'-UUCUUCUACAAACCGCUCC-3'. The siRNA inhibiting PDCD4 gene expression provides a novel technical scheme for studying functions of PDCD4 genes, andsensitivity of leukemia cells to chemotherapeutic drugs can be remarkably reduced by using siRNA to silence PDCD4.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to an siRNA inhibiting the expression of PDCD4 gene and its application in studying the function of PDCD4 gene. Background technique [0002] Programmed cell death 4 (PDCD4) is a new tumor suppressor gene discovered in recent years. Studies have reported that it plays a role in lung cancer, colorectal cancer, breast cancer, gastric cancer, pancreatic cancer, ovarian cancer, and glial cancer. Loss or low expression in various malignant tumor cells such as tumor and hepatocellular carcinoma. Chen et al. (Chen Y, Knosel T, Kristiansen G.J Pathol, 2003, (5):640-646.) studied 124 primary lung cancer samples of different subtypes, and the results showed that 83% of the tumor samples expressed PDCD4 protein Compared with the expression of PDCD4mRNA in normal lung tissue, the expression level of PDCD4mRNA in lung cancer tissue was significantly lower than that in normal lung tissue. Afonj...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/02G01N21/31
Inventor 费嘉谷景义朱雪娇骆小闯李育敏
Owner JINAN UNIVERSITY
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