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Method for extracting total flavonoids of bidens bipinnata and application of total flavonoids of bidens bipinnata (TFB) in preparation of medicine for treating rheumatoid arthritis

An extraction method and rheumatoid technology, applied in the field of medicine, can solve the problems that have not been reported in the literature of rheumatoid arthritis, etc., and achieve good social and economic benefits, rich original drug resources, and definite curative effect

Inactive Publication Date: 2012-10-10
WUXI NO 3 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching, there is no literature report on the treatment of rheumatoid arthritis with the total flavonoids of Guizhencao

Method used

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  • Method for extracting total flavonoids of bidens bipinnata and application of total flavonoids of bidens bipinnata (TFB) in preparation of medicine for treating rheumatoid arthritis
  • Method for extracting total flavonoids of bidens bipinnata and application of total flavonoids of bidens bipinnata (TFB) in preparation of medicine for treating rheumatoid arthritis
  • Method for extracting total flavonoids of bidens bipinnata and application of total flavonoids of bidens bipinnata (TFB) in preparation of medicine for treating rheumatoid arthritis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Extraction of total flavonoids from Bidens bidens:

[0020] The orthogonal experiment method was used to optimize the ethanol extraction process of the total flavonoids of Bidens bicolor. It was determined that the production process of ethanol extraction was to extract twice with an ethanol solution of 12 times the weight of Bidens bicolor and 80% mass concentration for 1 hour each time. And from the aspects of static adsorption-desorption capacity of total flavonoids, dynamic adsorption-desorption capacity, static adsorption kinetics process, etc., the seven domestic macroporous adsorption resins were studied, and the one most suitable for preparing total flavonoids of Bidens Macroporous adsorption resin HPD100. The process parameters of HPD100 purified total flavonoids were examined, and the process conditions were determined: the sample concentration of the extract was 1~1.5mg·mL -1 , With 2BV·h -1 The adsorption rate is high, and the maximum sample load is 4...

Embodiment 2

[0023] The extraction steps are as follows: take Bidens bidens, dissolve and extract 10 times the weight of Bidens bidens with 70% mass concentration of ethanol for 2 times, 1 hour each time; collect the extract and adsorb with macroporous adsorption resin HPD100; load the extract The concentration is 1mg·mL -1 , To 1BV·h -1 The adsorption rate is 3 mg·mL based on the total solute flavonoids. -1 Macroporous adsorption resin; after adsorption, first wash away impurities with 3BV water, and then use 3BV with a mass concentration of 70% ethanol to 3BV·h -1 Under these conditions, the macroporous adsorption resin can be used repeatedly for 3 times; after adsorption by the macroporous adsorption resin, the ethanol with a mass concentration of 70% is desorbed into a solution of Bidens flavonoids. The content and yield of total flavonoids in Bidens bidens total flavonoids solution were determined by colorimetry.

[0024] The total flavonoids in the Bidens vulgare total flavonoids solutio...

Embodiment 3

[0026] The extraction steps are: take Bidens bicolor, dissolve and extract with 90% ethanol of 14 times the weight of Bidens bicolor, extract twice, 1h each time; collect the extract and adsorb it with the macroporous adsorption resin HPD100; load the extract The concentration is 1.5mg·mL -1 , With 3BV·h -1 Adsorption is performed at the adsorption rate, and the loading amount is 5 mg·mL based on the total solute flavonoids -1 Macroporous adsorption resin; after adsorption, first wash away impurities with 5BV water, and then use 5BV with a mass concentration of 90% ethanol for 5BV·h -1 Under these conditions, the macroporous adsorption resin can be used repeatedly for 5 times; after adsorption by the macroporous adsorption resin, the ethanol with a mass concentration of 70%~90% is desorbed as Bidens In the flavonoid solution, the content and yield of the total flavonoids in the Bidens flavonoid solution were determined by colorimetry.

[0027] The total flavonoids in the Bidens vu...

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Abstract

The invention relates to a method for extracting the total flavonoids of bidens bipinnata (TFB) and an application of the TFB in the preparation of a medicine for treating rheumatoid arthritis. The method for extracting the TFB comprises the following steps of: taking the bidens bipinnata, dissolving by ethanol and extracting; collecting extract, and adsorbing by a macroporous resin; washing by water to remove impurities, and eluting by ethano; and measuring the content and the yield of the total flavonoids in the solution of the TFB by adopting the colorimetric method. By adsorbing and eluting under the condition, the macroporous resin can be reused. After adsorbing by the macroporous resin and desorbing by the ethanol, the solution of the TFB can be obtained. 80-160mg / kg of solution of the TFB is taken for treating rheumatoid arthritis. The new medicinal prospect of the TFB is excavated, and the effect of the TFB on treating rheumatoid arthritis is definite. The prepared TFB is taken orally and conveniently, has low toxic and side effects and can not cause harm to the human body after being taken for a long time.

Description

Technical field [0001] The invention relates to a method for extracting total flavonoids from Bidens bicolor and its application in preparing medicines for treating rheumatoid arthritis, and belongs to the technical field of medicine. Background technique [0002] Rheumatoid arthritis (RA) is a clinical syndrome of unknown etiology, with chronic polyarthritis as the main manifestation. It is currently considered to be a systemic autoimmune disease. The main feature is chronic, systemic, erosive peripheral joint synovitis, which mainly invades the synovial tissue, and then causes the destruction of articular cartilage, surrounding ligaments and bone. The disease has a global distribution. The prevalence rate in my country is 0.32% to 0.36%, which is lower than 1% of whites in European and American countries. It is one of the main causes of labor loss and disability in our population. The current treatment of RA is mainly: ①control joint and other tissue inflammation, relieve symp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P29/00A61P19/02
Inventor 李霞袁凤来赵懿清李成万贺春晖
Owner WUXI NO 3 PEOPLES HOSPITAL
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