Biological assay method for cotton aphid
An identification method and technology of aphids, applied in animal husbandry and other fields, can solve the problems of long identification time and labor-intensive identification technology, and achieve the effect of reasonable sequence of operation steps, simple method and easy operation
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[0022] (1) The test is carried out in the greenhouse. Fill a flat-bottomed U-shaped test tube (3cm in height and 2cm in diameter) with 1cm of agar with a concentration of 3wt‰-5wt‰, and seal the bottle mouth with a parafilm;
[0023] (2) Collect the second expanded leaf (with petiole) on the top of the cotton, and pass the petiole through the sealing film of the bottle mouth, so that the petiole is immersed in the water of the U-shaped bottle;
[0024] (3) Inoculate the natural population of cotton aphids collected from the cotton field on the leaves of step (2) with a brush, and place the U-shaped bottle in a light incubator (L:D=16:8);
[0025] (4) Use a transparent plastic cup (diameter 10cm, height 15cm) to buckle on the U-shaped test tube inserted with cotton leaves and inoculated with aphids in step (3), so that it is in a flat position to prevent the plastic cup from tipping over and falling cause aphids to escape;
[0026] (5) Investigate the number of live aphids on ...
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