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Biological assay method for cotton aphid

An identification method and technology of aphids, applied in animal husbandry and other fields, can solve the problems of long identification time and labor-intensive identification technology, and achieve the effect of reasonable sequence of operation steps, simple method and easy operation

Inactive Publication Date: 2012-10-10
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing conventional field or net room aphid identification technology is time-consuming and labor-intensive, so a short-time, simple and easy-to-operate indoor bioassay method for aphids is needed

Method used

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  • Biological assay method for cotton aphid
  • Biological assay method for cotton aphid
  • Biological assay method for cotton aphid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The test is carried out in the greenhouse. Fill a flat-bottomed U-shaped test tube (3cm in height and 2cm in diameter) with 1cm of agar with a concentration of 3wt‰-5wt‰, and seal the bottle mouth with a parafilm;

[0023] (2) Collect the second expanded leaf (with petiole) on the top of the cotton, and pass the petiole through the sealing film of the bottle mouth, so that the petiole is immersed in the water of the U-shaped bottle;

[0024] (3) Inoculate the natural population of cotton aphids collected from the cotton field on the leaves of step (2) with a brush, and place the U-shaped bottle in a light incubator (L:D=16:8);

[0025] (4) Use a transparent plastic cup (diameter 10cm, height 15cm) to buckle on the U-shaped test tube inserted with cotton leaves and inoculated with aphids in step (3), so that it is in a flat position to prevent the plastic cup from tipping over and falling cause aphids to escape;

[0026] (5) Investigate the number of live aphids on ...

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Abstract

The invention relates to the field of biological control, and in particular relates to a biological assay method for cotton aphid. The method comprises the following steps: (1) filling agar into a flat-base U-shaped test tube, sealing a bottle mouth with a sealing membrane and placing the bottle in a greenhouse; (2) collecting the second unfolded leaf at the top of cotton, and enabling the leaf stem to penetrate through the sealing membrane of the bottle mouth so as to immerse the leaf stem in water in the U-shaped bottle; (3) inoculating natural cotton aphid population collected from a cotton field on the leaf; and (4) cultivating and investigating the survival quantity of aphid, carrying out statistic analysis, and calculating aphid harm index and aphid harm index reduction rate as well as the resistant level to aphid. The method is short is time, simple and convenient, easy in operation and accurate and reliable.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a biological determination method for cotton aphids. Background technique [0002] Cotton suffers from a variety of pests and diseases throughout its life, with a loss of 15% to 20% due to pest damage all the year round, and a loss of more than 50% in serious years, or even no harvest. More than 900,000 tons of losses can be recovered every year through pest control. Due to the long-term, repetitive and sudden nature of agricultural biological disasters, especially the variety of cotton pests in my country, their wide distribution, complex fluctuations, and high frequency of disasters, coupled with the fact that my country's agricultural planting structure and farming system The continuous changes and the influence of abnormal climate make the prevention and control of cotton diseases and insect pests in my country face new challenges. [0003] With the development of biotechnol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 雒珺瑜崔金杰马艳辛慧江王春义张帅吕丽敏李春花吴冬梅
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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