Construction method of tilapia fillet quality evaluation model based on proteins and enzymes in muscle
A technology for quality evaluation and tilapia fillets, which is used in material inspection products, testing food, etc., can solve problems such as differences and deviations in subjective judgments, consuming a lot of manpower and time, and limitations of grading and viewing factors, and achieve prediction. Fast speed, broad development and application prospects, high-confidence effects
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Embodiment 1
[0021] Example 1: Freshly slaughtered tilapia fillets
[0022] The specific construction process of this embodiment includes the following steps:
[0023] (1) The change of protein in fish muscle during storage is the reason for the change of tissue shape, elasticity, smell and appearance of muscle. ATP enzymes can reflect the denaturation of protein, so Ca in tilapia fillets is selected. 2+ -ATPase activity, Mg 2+ -ATPase activity, salt-soluble protein content, and sulfhydryl content are used as quality evaluation indicators;
[0024] (2) Use the commonly used fuzzy analytic hierarchy process to determine the quality evaluation indicators of tilapia, construct a comparison matrix for the selected quality evaluation indicators, calculate the weight vector and do a consistency test, and the weight value of the above indicators is obtained: Ca 2+ - The weight value of ATPase activity is 0.3423, Mg 2+ - The weight value of ATPase activity is 0.1449, the weight value of salt-so...
Embodiment 2
[0028] Example 2: Tilapia fillets stored at 4°C for 2 days
[0029] The construction steps of this example are the same as those of Example 1, the difference is that tilapia fillets refrigerated for 2 days are used to collect index data, and Ca 2+ -ATPase activity is 1.743μmolPi / mg prot / hour, Mg 2+ -ATPase activity is 2.701μmol Pi / mg prot / hour, salt-soluble protein content is 5.868mg / g, thiol content is 0.216mmol / mol. Substituting the value into the quality evaluation model, the quality score is 3.14.
Embodiment 3
[0030] Example 3: Tilapia fillets stored at 4°C for 9 days
[0031] The construction steps of this example are the same as those of Example 1, the difference is that tilapia fillets refrigerated for 9 days are used to collect index data, and Ca 2+ -ATPase activity is 1.307μmolPi / mg prot / hour, Mg 2+ -ATPase activity is 2.133μmol Pi / mg prot / hour, salt-soluble protein content is 2.876mg / g, thiol content is 0.109mmol / mol. Substituting the value into the quality evaluation model, the quality score is 1.82.
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