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DNA (Deoxyribose Nucleic Acid) sequencer

A technology of a sequencer and a sequencing chip, applied in the field of DNA sequencing, can solve the problem of low work efficiency and achieve the effect of improving the efficiency of DNA sequencing

Active Publication Date: 2012-10-03
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The inventor found that the DNA sequencer in the prior art has only one reaction chamber, and one instrument can only conduct one experiment at the same time, and the work efficiency is not high

Method used

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Embodiment Construction

[0086] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

[0087] like figure 1 , figure 2 and image 3As shown, the present invention provides a DNA sequencer, including a support platform 101, a plurality of shock absorbers 103 and a shock absorber 102 connected to the support platform 101 through a plurality of shock absorbers 103; The reaction chamber assembly 104, the reaction chamber assembly 104 includes a support frame 84 vertically arranged on the shock absorbing plate and a plurality of reaction chambers arranged side by side on the support frame 84; in this embodiment, four reaction chambers are taken as an example for illustration . The CCD camera 110 used to collect the optical signal generated by the DNA sequencing reaction in the reaction chamber; used to support the CCD camera 110 and drive the CCD camera 110 to approach or...

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Abstract

The invention discloses a DNA (Deoxyribose Nucleic Acid) sequencer which comprises a supporting table, a plurality of vibration dampers, a vibration damping plate, a reaction bin assembly, a CCD (Charge Coupled Device) camera, a two-dimensional regulation supporting device and a medicament supply assembly, wherein the vibration damping plate is connected with the supporting table by a plurality of vibration dampers; the reaction bin assembly is fixedly arranged on the vibration damping plate and is used for performing the DNA sequencing reaction; the CCD camera is used for acquiring an optical signal; the two-dimensional regulation supporting device is used for supporting the CCD camera; and the medicament supply assembly is arranged on the supporting table and is used for providing reagents and buffer solution for the reaction bin assembly. According to the DNA sequencer disclosed by the invention, by the arrangement of a plurality of reaction bins and the matching of the two-dimensional regulation supporting device capable of carrying out two-dimensional regulation and the medicament supply assembly capable of supplying the reagents for a plurality of reaction bins, the aim of simultaneously performing a plurality of reactions is fulfilled, a plurality of samples can be simultaneously sequenced and the DNA sequencing efficiency is greatly improved.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing, in particular to a DNA sequencer. Background technique [0002] In the field of DNA sequencing technology, pyrosequencing technology (pyrosequencing) is a new enzyme cascade sequencing technology developed by Nyren et al. Comparable, but the speed is greatly improved. [0003] Pyrosequencing technology is an enzyme cascade chemiluminescent reaction in the same reaction system catalyzed by four enzymes. The principle of pyrosequencing technology is: after the primer anneals to the template DNA, the four enzymes DNA polymerase (DNA polymerase), ATP sulfurylase (ATP sulfurylase), luciferase (luciferase) and adenosine triphosphate (Apyrase) Under the synergistic effect of the primer, the polymerization of each dNTP on the primer is coupled with the release of an optical signal, and the purpose of real-time determination of DNA sequence is achieved by detecting the release and intensity of li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34
Inventor 任鲁风王绪敏李运涛周晓光袁丽娜冯玉臣秦奕韩伟静谷岚滕明静俞育德于军
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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