Method for producing L-alanine by bioconverting L-aspartic acid by free cell process

A technology of free cells and aspartic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as side reactions, large equipment investment, and complicated separation

Active Publication Date: 2013-10-02
YANTAI HENGYUAN BIOENGINEERING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The product concentration of microbial fermentation method is low, the production cycle is long, the equipment investment is large, there are side reactions, the separation is complicated, and the technical risk is high, so it is not suitable for large-scale production of L-alanine

Method used

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  • Method for producing L-alanine by bioconverting L-aspartic acid by free cell process
  • Method for producing L-alanine by bioconverting L-aspartic acid by free cell process

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Experimental program
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Embodiment 1

[0051] culture medium

[0052] Activated slant medium (g / L): sodium glutamate 10.0, peptone 8.0, sodium chloride 5.0, magnesium sulfate 1.0, potassium dihydrogen phosphate 0.5, yeast extract 5.0, beef extract 4.0, agar 20.0, adjust pH 7.0- 7.2.

[0053] Shake flask seed medium (g / L): sodium glutamate 15.0, peptone 10.0, corn steep liquor dry powder 5.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.25, dipotassium hydrogen phosphate 0.12, adjust pH to 7.0.

[0054] Fermentation medium (g / L): peptone 12.0, corn steep liquor powder 4.0, sodium glutamate 12.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.30, dipotassium hydrogen phosphate 0.12, adjust the pH to 7.0 with sodium hydroxide solution.

[0055] Take a ring of Comamonas testosteroni HY-08D slant strain and connect it to the slant medium, culture at 35°C±1°C for 18h, then take a ring of strain and connect it to 150mL shake flask medium (inoculate several bottles), 150rpm , and cultivated at 28°C for...

Embodiment 2

[0057] culture medium

[0058] Activated slant medium (g / L): sodium glutamate 12.0, peptone 10.0, sodium chloride 7.0, magnesium sulfate 1.2, potassium dihydrogen phosphate 0.7, yeast extract 7.0, beef extract 3.0, agar 20.0, adjust pH 7.0- 7.2.

[0059] Shake flask seed medium (g / L): sodium glutamate 17.0, peptone 12.0, corn steep liquor dry powder 7.0, sodium hydroxide 8.0, magnesium sulfate heptahydrate 0.35, dipotassium hydrogen phosphate 0.08, adjust pH to 7.0.

[0060] Fermentation medium (g / L): peptone 15.0, corn steep liquor powder 5.0, sodium glutamate 15.0, sodium hydroxide 6.0, magnesium sulfate heptahydrate 0.10, dipotassium hydrogen phosphate 0.15, adjust the pH to 7.0 with sodium hydroxide solution.

[0061] Take a ring of Comamonas testosteroni HY-08D slant strain and connect it to the slant medium, culture at 25°C±1°C for 40h, then take a ring of strain and connect it to 150mL shake flask medium (inoculate several bottles), 100rpm , and cultured at 32°C for ...

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Abstract

The invention relates to a method for producing L-alanine by bioconverting L-aspartic acid by a free cell process, which comprises the following steps: bioconverting L-aspartic acid with Comamonas testosteroni HY-08D of self-screened high-yield L-aspartate-beta-decarboxylase (EC 4.1.1.12, ASD or ADL) to produce L-alanine; and filtering, concentrating, crystallizing and drying to obtain the finished L-alanine product. The invention can enhance the production efficiency of L-alanine and lower the production cost.

Description

technical field [0001] The invention relates to a production method of L-alanine, in particular to a method for producing L-alanine by bioconverting L-aspartic acid with a free cell method. Background technique [0002] L-alanine, also known as L-alpha-alanine (L-Alanine, L-Ala). L-alanine is the basic unit of protein, one of the 21 kinds of amino acids that make up human protein, and one of the amino acids that are closely related to sugar metabolism in the living body and have a large demand. Molecular formula is C 3 h 7 NO 2 , molecular weight 89.09, its structure is as follows: [0003] [0004] L-alanine structure [0005] The appearance of L-alanine product is white crystalline powder, odorless, sweet taste, soluble in water, slightly soluble in ethanol, insoluble in ether and propanol, sublimation begins above 200°C, melting point 297°C (decomposition), specific gravity 1.401, the pH value of 5% aqueous solution is 5.5-7.0. [0006] In recent years, with the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P13/06C12R1/645
Inventor 马玉岳
Owner YANTAI HENGYUAN BIOENGINEERING CO LTD
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