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Method for producing ethanol by fermenting straw hydrolysate

A technology of hydrolyzate and straw, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve problems such as affecting the fermentation efficiency of fermenting microorganisms

Inactive Publication Date: 2012-09-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The invention aims to solve the technical problem that the straw saccharification and fermentation process affects the fermentation performance of fermentation microorganisms, and provides a method for producing ethanol by fermenting straw hydrolyzate

Method used

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Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0010] Specific implementation mode one: the method for producing ethanol by fermenting straw hydrolyzate in this implementation mode is carried out according to the following steps:

[0011] 1. Treat the straw with steam explosion at 180℃~210℃ for 4min~8min, then add water until the solid content is 18%~23%, adjust the pH value to 4.6~5.5, then sterilize at 121℃ for 20min, add cellulase , biological antidote FYL, the mass ratio of cellulase and steam-exploded stalk is 0.1:1, the mass ratio of biological antidote FYL to steam-exploded straw is 0.1:1, at a temperature of 50°C, stirring Saccharification for 48 hours at a speed of 150 rpm to obtain straw hydrolyzate;

[0012] 2. Add manganese chloride tetrahydrate, zinc sulfate heptahydrate, nickel chloride hexahydrate, cobalt chloride octahydrate, pantothenic acid, inositol and vitamin B to the straw hydrolyzate 1 , then adjust the pH value to 5.0~5.5 to obtain a mixed solution, the concentration of manganese chloride tetrahydr...

specific Embodiment approach 2

[0016] Embodiment 2: This embodiment differs from Embodiment 1 in that the pH value is adjusted to 5.1-5.4 in step 2. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0017] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is that the pH value is adjusted to 5.3 in step two. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for producing ethanol by fermenting straw hydrolysate, in particular to a method for improving the fermentation efficiency of ethanol, aiming at solving the technical problem that the fermentation efficiency of fermentation organisms is influenced by a straw diastatic fermentation technology. The method comprises the steps of: adding manganese chloride tetrahydrate, zinc sulfate heptahydrate, nickel chloride hexahydrate, cobalt chloride octahydrate, pantothenic acid, inositol and vitamin B1 into the straw hydrolysate, and adjusting the pH value to be 5.0-5.5 to obtain mixed solution; adding yeast into the mixed solution until the concentration of the yeast is 1.5g / L; and then, carrying out fermentation for 48-72h under the condition that the temperature is 32-40 DEG C, and the stirring speed is 150rpm to finally obtain ethanol. The yield of ethanol fermented at the temperature of 40 DEG C and the pH value of 5.0-5.5 is 2-3 times of that of ethanol which is directly fermented without being added with the trace elements (including manganese chloride tetrahydrate, zinc sulfate heptahydrate, nickel chloride hexahydrate, cobalt chloride octahydrate, pantothenic acid, inositol and vitamin B1).

Description

technical field [0001] The invention relates to a method for improving the efficiency of fermenting ethanol. Background technique [0002] With the rapid growth of ethanol consumption, the first-generation ethanol based on grains / sugars can no longer meet the market demand, so the world has set off an upsurge in the development of the second-generation ethanol—cellulosic ethanol. However, due to the high cost of cellulosic ethanol, it has not been industrialized until now. One of the reasons for the high cost of cellulosic ethanol is that the fermentation efficiency of the fermentation microorganisms on the cellulose hydrolyzate is not high, which eventually leads to the low ethanol concentration in the fermentation liquid, which requires a lot of energy consumption in the distillation process. In the existing cellulose ethanol production, the six-carbon sugar ethanol fermentation process in the cellulose hydrolyzate is almost completely copied from the grain-based ethanol ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/10
Inventor 冯玉杰于艳玲李梓木
Owner HARBIN INST OF TECH
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