Stem cell cryopreserving box and cryopreservation method

Abstract

The invention discloses a stem cell cryopreserving box and a cryopreservation method. The stem cell cryopreserving box comprises a box body, a box cover and baffle plates, wherein an opening is arranged at the top part of the box body, and pressure adjusting and fixing devices are arranged outside two opposite side walls on the box body; a plurality of collection bags and a plurality of baffle plates are arranged in the box body alternately, and the baffle plates are located at the topmost part of the alternate arrangement of the collection bags and the baffle plates; and the box cover is located at the top part of the box body and is connected with the pressure adjusting and fixing devices on the side walls of the box body by fasteners. Compared with the prior art, when the stem cell cryopreserving box and the cryopreservation method are used, stem cell samples of the same patient can be placed in one box body, and the box cover can press the collection bags into a flat shape, so that stem cells can be uniformly cooled; each box body, as an independent storage unit, is convenient to take and use; and when the box body is taken and used, the operation is simple, and the consumed time is shorter, so that the refrigerator door opening time is shortened, and the temperatures of other box bodies are slightly influenced.

Description

technical field [0001] The present application relates to the technical field of medical equipment, in particular to a stem cell cryopreservation box and a cryopreservation method. Background technique [0002] Stem cells are usually stored in a low-temperature refrigerator / liquid nitrogen tank at -85°C or -196°C according to the required storage period. For stem cells that have a short period from collection to use (<6 months), usually use a deep-low temperature refrigerator to store them at -85°C. At present, there is no unified operating standard for the cryopreservation of this type of stem cells, and the storage boxes selected by each unit are not the same. Since the general hard plastic (referring to the storage box body) is easy to freeze and crack at deep low temperature, most of them Simply use some box-shaped paper materials to temporarily hold the stem cells collected in the blood transfusion bag. Above-mentioned preservation method has following shortcoming ...

AI Technical Summary

Problems solved by technology

[0003] (1) Stem cells of multiple patients are often stored in a deep-low temperature refrigerator, and the stem cells of different patients can only be stacked and stacked in disorder, and the position can be marked poorly, making the inside of the refrigerator appear chaotic;

[0004] (2) Different storage boxes are often in a freeze-thaw bonded state under low temperature environment. When taking out the storage box located in the lower layer, an external force is required to separate the two bonded storage boxes. The operation is cumbersome, and this operation also makes the If the refrigerator door is opened for too long, the temperature in the refrigerator cannot be kept constant, which affects the freezing effect of stem cells in the entire refrigerator;

[0005] (3) Stem cells are usually collected in plastic collection bags. When storing in the refrigerator, in order to keep the cooling even, it is often necessary to press a heavy object on the collection bag to keep the collection belt in a flat state. Usually, the choice of this heavy object is also There are all kinds of strange things, such as: thick books, ice cubes, etc. This kind of irregular operation has serious safety hazards, and it will also affect the quality of stem cell cryopreservation due to the weight being too light or too heavy

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