Method for representing gas-liquid contact type cigarette full smoke exposure by using light absorption value

A technology of gas-liquid contact and light absorption value, applied in the direction of color/spectral characteristic measurement, etc., can solve problems such as no public reports, and achieve the effect of simple operation

Active Publication Date: 2012-07-18
YUNNAN RES INST OF TOBACCO SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Through literature search, there is no public report identical with the present invention

Method used

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  • Method for representing gas-liquid contact type cigarette full smoke exposure by using light absorption value
  • Method for representing gas-liquid contact type cigarette full smoke exposure by using light absorption value
  • Method for representing gas-liquid contact type cigarette full smoke exposure by using light absorption value

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Embodiment Construction

[0023] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0024] 1. Experimental material: the Chinese hamster ovary cell (Chinese Hamster Ovary Cell, CHO cell) that embodiment relates to is the cell used in laboratory routine experiment, buys from domestic cell preservation institution; Salmonella typhimurium test bacterial strain TA98 and TA100 are bacterial recovery Bacteria dedicated to mutation test (Ames test) were purchased from Molecular Toxicolog, Inc., USA.

[0025] 2. Main experimental equipment: a device for exposure to cigarette smoke ZL201120063251.6, a commercially available double-beam ultraviolet spectrophotometer TU-1901, a linear smoking machine and a water bath.

[0026] 3. Experimental principle: According to the CHO cells exposed to different smoking volumes or the Ames test, the bacterial suspension has an absorption peak at 253-288nm, and its absorbance value has a corresponding change...

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Abstract

The invention relates to a method for representing gas-liquid contact type cigarette full smoke exposure by using light absorption value, belonging to the technical field of safety evaluation of cigarette smoke. The method comprises the following steps of: 1, placing two parts of a given amount of cell suspension or bacterial suspension special for Salmonella typhimurium / mammals microsomal enzyme test (Ames test) after being exposed to the clean air, into ultraviolet spectrophotometer cuvettes, then respectively arranging the ultraviolet spectrophotometer cuvettes into inner and outer sample cells and adjusting the base line in the wavelength range of 200nm-600nm; 2, taking out the cuvette in the outer sample cell and adding an equal amount of cell suspension or bacterial suspension to be detected after being exposed to the smoke, into the cuvette and then placing the cuvette into the sample cell, scanning the cuvette by spectrum with wide band of 1nm in the wavelength range of 200nm-600nm to obtain a spectrum curve and a light absorption value; and 3, repeating the step 2, scanning the suspensions after being exposed to different smoke amounts by spectrum, so as to obtain the spectrum curves and light absorption values of the suspensions exposed to different smoke amounts in 253nm-288nm. The method is simply operated and the exposure under the gas-liquid contact type cigarette full smoke exposure is conveniently, quickly and qualitatively represented by the spectrum curves and the light absorption values.

Description

Technical field: [0001] The invention relates to a method for qualitatively characterizing the total smoke exposure of gas-liquid contact cigarettes by using light absorption value, and belongs to the technical field of cigarette smoke safety evaluation. Background technique: [0002] The full-smoke exposure method can truly reflect the physical and chemical characteristics of fresh smoke, and relatively simulates the exposure of human cells to smoke during smoking. high attention of personnel. However, the control and detection of smoke exposure in this method is a research difficulty, and scientists from various countries are working hard to tackle the problem. [0003] The current real-time detection method of smoke exposure is to measure the particle phase components and gas phase components of the smoke respectively. The Institute of Toxicology and Experimental Medicine of the German Fraunhofer Research Association used light scattering spectroscopy for the determinat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/33
Inventor 夭建华曾婉俐米其利朱洲海李雪梅缪明明
Owner YUNNAN RES INST OF TOBACCO SCI
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