Device for acquiring multispectral microscopic image

Abstract

The invention relates to a device for acquiring a multispectral microscopic image. The device for acquiring the multispectral microscopic image comprises a microscope, an adjustable band-pass optical filtering device and a camera which are arranged with the same optical axis, wherein the camera is electrically connected with a computer; the adjustable band-pass optical filtering device is connected with a motor; the motor is controlled by a controller and a computer to rotate or swing to drive the adjustable band-pass optical filtering device to swing in an optical path between a light source of the microscope and the camera; and the adjustable band-pass optical filtering device is one adjustable band-pass optical filtering sheet or two band-pass optical filtering sheets assembled at a certain included angle. Compared with the prior art, the invention has the benefits that: 1) the device for acquiring the multispectral microscopic image has a simple structure and is easy to implement and convenient to operate; 2) the device for acquiring the multispectral microscopic image has high spectral transmittance; 3) the included angle of the adjustable band-pass optical filtering sheet and the optical axis can be continuously changed in the range of 0 to 60 degrees, the expansibility is high and the wavelength can be changed; and 4) the whole system is low in manufacturing cost.

Description

technical field [0001] The invention belongs to the technical field of microscopes, and in particular relates to a multispectral microscopic image acquisition device. Background technique [0002] Compared with ordinary microscopes, multispectral microscopy can obtain one-dimensional information, that is, spectral information, and can provide more accurate quantitative analysis. Therefore, it has important roles in the fields of clinical medicine, pathology, histology, immunohistochemistry, pharmaceutical engineering, and materials science. Value. [0003] Current multispectral microscopes generally use gratings, electrically tunable filter devices (LCTF or AOTF), and color filters to obtain spectral information. For example, Chinese patent ZL200410017031.4 uses gratings to obtain spectral information. The filter device obtains spectral information, although both methods can obtain continuous microscopic images with high spectral resolution, but the cost is relative...

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Problems solved by technology

[0003] Current multispectral microscopes generally use gratings, electrically tunable filter devices (LCTF or AOTF), and color filters to obtain spectral information. For example, Chinese patent ZL200410017031.4 uses gratings to obtain spectral information. The optical filter device obtains spectral information, although both methods can obtain continuous microscopic images with high spectral resolution, but the cost is relatively high

[0004] Patent WO2008074019A2 discloses a method using a color filter array, which can obtain multispectral data of a limited number of bands. Although the structure is compact, the spatial resolution will be reduced; the more bands, the more the spatial resolution will be reduced, and After the color filter array is determined, its spectral band is also determined, it cannot be replaced at will, it can only be used for special use, and the expandability is not strong

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