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Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit

A detection method and urease technology are applied in the direction of using chemical indicators for analysis, etc., which can solve the problems of low detection accuracy, unintuitive detection results, complicated testing procedures, etc., and achieve the effect of a simple detection method.

Active Publication Date: 2013-11-20
李雄
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  • Abstract
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  • Application Information

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Problems solved by technology

The existing technical methods generally have the disadvantages of complex testing procedures, inconvenient operation, low detection accuracy, unintuitive detection results, and low safety factor.

Method used

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  • Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit
  • Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit
  • Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit

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Embodiment Construction

[0016] In order to better understand the present invention, the present invention will be further described below in conjunction with specific embodiments, but the scope of protection claimed by the present invention is not limited to the scope described in the embodiments.

[0017] The method for detecting rapid urease in tongue coating by pH ethanol method of the present invention includes the following steps:

[0018] A. Use a pH test paper to measure the pH of oral saliva; the pH test paper is a precise pH test paper with a detection range of 5.5 to 9.0; produced by Shanghai Sanaisi Reagent Co., Ltd.;

[0019] B. Put 3 to 4 drops of 95% ethanol solution into the fast urease reagent cup, and mix the reagent particles attached to the bottom of the cup with the ethanol solution with a toothpick; the fast urease reagent cup is a fast diagnostic reagent for Helicobacter pylori Cup, produced by Ningbo Meikang Biological Technology Co., Ltd.;

[0020] C. Use a cotton swab to wipe off the...

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Abstract

The invention discloses a rapid detection method of urease in tongue coating by using a pH value-ethanol method and a kit. The detection method comprises the following steps: A. measuring pH value of oral saliva with a pH test strip; B. dropwise adding 3-4 drops of 95% ethanol solution into a rapid urease reagent cup, and fully mixing reagent particles attached at the cup bottom and the ethanol solution with a toothpick; C. taking the tongue coating at the back of the tongue and placing the tongue coating in the reagent cup, fully mixing, dropwise adding additional 3-4 drops of dissolving solution into the reagent cup following standing for 5 minutes, fully mixing, standing for 5-10 minutes, and observing the color change; and D. measuring with the pH test strip if the color is changed into red, comparing the result with the pH value of the oral cavity, and thereby judging the result. The kit comprises a kit body, and the rapid urease reagent cup, a soft plastic bottle filled with 1-10ml of the dissolving solution and the 95% ethanol solution, pH precise test strips with detection range of 5.5-9.0, a packet of cotton swabs and a packet of toothpicks are arranged in the kit body. The detection method provided by the invention is simple and rapid, causes no wound and has low cost.

Description

Technical field [0001] The invention relates to a method for detecting rapid urease in the tongue coating by the pH value alcohol method, and also relates to a kit for the method for detecting rapid urease in the tongue coating by the pH value alcohol method. Background technique [0002] Helicobacter pylori is closely related to a variety of diseases in the stomach and outside the stomach. Because of its pathogenicity and tumor-related characteristics, it has received extensive attention and continuous in-depth research. The prior art methods generally have the disadvantages of complicated testing procedures, inconvenient use and operation, low detection accuracy, unintuitive detection results, and low safety factor. Summary of the invention [0003] The purpose of the present invention is to overcome the deficiencies in the prior art, and provide a simple, non-invasive, rapid, and inexpensive method for detecting rapid urease in the tongue coating with a pH value ethanol method,...

Claims

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Application Information

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IPC IPC(8): G01N31/22
Inventor 李雄
Owner 李雄
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