Method for detecting distribution of lateral line canals of fish

Abstract

The invention relates to a method for detecting distribution of lateral line canals of fish, which relates to the technology of detection of sensory organs of the fish. The invention aims to solve the technical problems to provide the detecting method, which has the advantages that: the operation is simple and convenient, dye injected into the lateral line canals is not easy to fade, and the shapes and the structures of the lateral line canals are easy to observe. The method has the technical scheme that a fish sample is adopted, and is characterized by comprising the following steps of: using 10 percent of formalin to fix the fish sample for 48h, or mixing commercially available 10ml of 37 percent to 40 percent of formaldehyde solution and 95 ml of distilled water to fix the fish sample for 48h; washing the fish sample by using tap water for overnight, and removing formalin residues of the fish sample; putting the fish sample into formic acid solution with the concentration of 5 percent, and carrying out decalcifying for 12h so as to soften bone tissue; and injecting the dye from a temporal upper tube by using 5ml of medical injector, wherein the dye is a red mixture, which is prepared by the following steps of: pulverizing 5g of ABS (acrylonitrile-butadiene-styrene) plastic, adding into 60ml of acetone, mixing uniformly, and adding 0.5g of solvent red.

Description

Technical field: [0001] The invention relates to fish sensory organ detection technology. Background technique [0002] The lateral canal system of fish is a sensory organ derived from the skin, which has the functions of sensing water flow, water pressure, water temperature, and hearing; it is unique to fish and aquatic amphibians. The commonly used methods for detecting the distribution of lateral line ducts in fish mainly focus on the following two aspects: after the sample is fixed in formalin, the skin is cut open along the longitudinal axis at the side edge of the fish with a scalpel to separate the skin from the muscle, Look for milky white lateral line tubes on the surface; for formalin-soaked specimens, use H 2 o 2 After fading, inject Delafield's hematoxylin stain from the supraorbital canal into the lateral line tube through the perfusion device. This procedure requires the specimen to be put into running water for operation, and the contrast between the stained...

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to provide a method that is easy to operate, and the dye injected into the side line pipe is not easy to fade in view of the shortcomings of the prior art that the process is cumbersome, the structure of the side line pipe is easily damaged, and the dye injected into the side line pipe is easy to fade. An easy-to-observe detection method for the shape and structure of side pipelines