Method for inhibiting generation of aflatoxin by using D-glucal
A technology of aflatoxin and Aspergillus flavus, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.
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[0023] Example 1, D-glucal inhibits aflatoxin production
[0024] Aspergillus flavus (CGMCC No. 3.2890) was purchased from the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences; the initiation culture of the strains: a small amount of the strains frozen in glycerol at -80°C was evenly spread on the PDA medium for activation, After culturing at 37°C for 4 days under aerobic conditions, green spores can be observed.
[0025] Spore counting: (nephelometric method) wash Aspergillus flavus spores repeatedly with 0.05% Tween20 water, the spores are finally in the form of dispersed particles, dilute in multiples, count with a hemocytometer respectively, and take 2.5ml and pour it into a cuvette, at OD600nm Measure the light absorption at , and establish a standard curve for the concentration of spores, and only need to measure the OD for the spores to be tested 600nm Light absorbance, spore number was determined by calculation. Dilute with Twee...
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