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Immunochromatography reagent strip and sealing agent composition used for same

A technology of immunochromatography and composition, which is applied in the field of immunochromatography, can solve the problems of protein line dispersion, decrease in adsorption capacity, and decrease in protein quality, and achieve the effect of reducing background increase, long-term use, and easy use

Active Publication Date: 2012-04-25
SINOCARE
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Problems solved by technology

[0007] After the NC membrane is treated by the above methods, the originally hydrophobic membrane becomes hydrophilic, and the adsorption capacity of biomacromolecules on the NC membrane is greatly reduced. However, after the above series of treatments, the NC membrane also brings many problems. : If the position of the protein immobilized on the NC membrane is changed due to the addition of surfactant, the protein line appears to be diffused, and the amount of protein originally immobilized on the NC membrane is reduced
At the same time, the problem of blocking agent protection after long-term storage of test strips has not been reported at present, because blocking agents are biologically active and easy to inactivate; therefore, some protein protection agents need to be added to achieve long-term preservation of test strip performance

Method used

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  • Immunochromatography reagent strip and sealing agent composition used for same

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specific Embodiment

[0039] 1. Comparison of protein blocking effect

[0040] Particle labeling: First, the fluorescent latex particles are activated by EDC and NHS, and then the particles react with monoclonal antibodies against human serum albumin. After centrifugation and elution, the particles labeled with antibodies are suspended in the following 5 different proteins In the blocking solution: 1. Blank group, 2. 0.5% gelatin blocking solution; 3. 0.5% BSA blocking solution; 4. 0.5% casein blocking solution; 5. 0.5% skimmed milk powder blocking solution; 6. Casein treatment solution; 7.1% methylated bovine serum albumin.

[0041] The preparation method of the casein treatment solution: firstly prepare a 0.1 mol / l glycine-hydrochloric acid buffer solution with a pH value of 6.5, then dissolve casein in the buffer solution, and finally autoclave treatment to obtain the casein treatment solution.

[0042] Test strip production: first paste the NC film on the plastic board, and then scribe the line on th...

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Abstract

The invention provides a sealing agent composition used for an immunochromatography reagent strip, which comprises protein, protein protective agent, surface active agent and first buffer solution, wherein the protein is casein, casein treatment solution, methylation bovine serum albumin (BSA) and human serum albumin; and the protein protective agent is saccharide. The sealing agent composition provided by the invention is capable of effectively reducing the background of the immunochromatography reagent strip, and is long in service life and high in detection sensitivity. The invention also provides the immunochromatography reagent strip.

Description

Technical field [0001] The invention relates to immunochromatography technology, in particular to a blocking agent composition for immunochromatography test strips and an immunochromatography test strip. Background technique [0002] With the development of immunodiagnostic technology, the comprehensive application of various raw materials and various detection methods, the technology is becoming more and more compact, simple, fast, and highly accurate. Immunochromatography technology is a very important part of this development. It has changed the development direction of this technology in space and time. In space, it has moved from the previous laboratory to the home. In time, it has The genius can shorten the test result to a few minutes. Thus greatly promoted the rapid development of immunodiagnostic technology. [0003] Immunochromatography technology uses the reaction principle of high specificity and high sensitivity of antigen and antibody, and detects the antigen (or an...

Claims

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Application Information

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IPC IPC(8): G01N33/558
Inventor 聂海龙
Owner SINOCARE
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