Method for distinguishing back muscles from muscles of three parts of male cyprinus carpio
A muscle, back technology, used in biological testing, material inspection products, etc.
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[0019] The present invention will be further described below in conjunction with embodiment.
[0020] Materials: GAPDH protein antibody, mouse secondary antibody, and rabbit secondary antibody were purchased from Jill Biochemical (Shanghai) Co., Ltd.
[0021] 1. The five-tailed male carp was taken from the Yixing Breeding Base of Wuxi Freshwater Fishery Research Center of the Chinese Academy of Fishery Sciences. The back muscles, abdominal muscles and tail muscle tissues were taken, and they were quickly frozen in liquid nitrogen and stored in a -80°C refrigerator for easy generalization. RNA extraction. The extraction of total RNA was carried out according to the operating instructions of RNAiso Plus, and dissolved in DEPC water after the extraction was completed. The extraction of RNA was detected by agarose gel electrophoresis, the concentration of RNA was detected by spectrophotometry, and then the RNA was reverse-transcribed into cDNA with a reverse transcription kit for...
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