Method for constructing mouse model for drug research

Abstract

The invention discloses a method for constructing a mouse model for drug research, which is particularly applied to construction of a mouse model for researching drug with a radioactive label. The constructing method disclosed by the invention is characterized in that: an inferior vena cava of a mouse is adopted as a drug administration part when the mouse is in narcosis. The method disclosed by the invention has the advantages that the operation is simple, convenient and easy, the drug absorption is fast, the drug can be effectively prevented from exposing, and the like, and the drug can be dispersed to the whole body of the mouse or target points to be inspected after the drug administration is carried out for 30min-60min.

Description

technical field [0001] The present invention relates to a method for constructing a mouse model, in particular to a method for constructing a mouse model for drug research, and the present invention is especially suitable for the construction of a mouse model with radiolabeled drug research. Background technique [0002] In all kinds of modern basic medical research, in order to observe the pharmacodynamics and toxicology of drugs, new compounds, etc., or in the research of the etiology, pathogenesis and pathology of diseases, in order to observe the changes in animal body functions, metabolism and morphology, Usually, it is necessary to adopt a certain way of administration to experimental animals, and the method of injecting mice is the most used at present. The common methods are intraperitoneal injection, intravenous injection, subcutaneous injection and intradermal injection. , intramuscular injection, etc. Among them, intraperitoneal injection is a common way of admin...

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Problems solved by technology

Among them, intraperitoneal injection is a common way of administration, especially during anesthesia. Its advantage is that it is easy to operate, but the drug is absorbed slowly and is easy to lose; the drug absorption rate of subcutaneous injection and intradermal injection is slower than that of intraperitoneal injection. And it is easy to cause drug leakage during injection; intravascular drug injection in mice is usually carried out through veins, and mouse tail vein injection is a method often used for intravenous injection, but the tail vein is thin, and the injection of drugs often cannot be performed due to operational reasons. Into the blood vessel or overflow from it, causing environmental pollution, so this method requires high skill and proficiency of the operator, the operation is cumbersome, and the tail vein is a peripheral circulation, the circulation is slow, and the drug is dispersed after injection into the animal body. The time is also slow, which is not conducive to the diffusion of drugs; the method of intramuscular injection is not suitable for the injection of mice due to their small size and less muscles

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