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Method for rapidly evaluating aphid resistance effect of transgenic insect-resistant cotton

A technology of genetically modified and insect-resistant cotton, which is applied in measuring devices, material analysis by electromagnetic means, instruments, etc., to achieve the effects of simple method, good repeatability and accurate evaluation method

Inactive Publication Date: 2012-01-25
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned methods for evaluating the anti-aphid effect of transgenic insect-resistant cotton mainly have the following disadvantages: (1) A large number of aphids are required for the measurement, usually hundreds or thousands of aphids head aphids; (2) The measurement period is too long, usually dozens of days or several months

Method used

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  • Method for rapidly evaluating aphid resistance effect of transgenic insect-resistant cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] ⑴ Preparation of equipment and materials to be tested

[0026] The detection instrument is a 4-channel insect direct current piercing potential (electrical penetration graph). The two cotton lines to be tested are A1 and A2, and the growth period is the seedling stage with 4 true leaves. Connect the back plate of the cotton aphid to the gold wire with water-soluble conductive silver glue, put it on the cotton seedling leaves and adapt to it for 1 hour, and then connect the other end of the gold wire electrode to the amplifier of the piercing and suction potentiometer. Only one aphid without wings can be placed on each cotton seedling to be tested, and only one aphid can be connected to each channel of the potentiometer amplifier, and both the cotton seedling and the cotton aphid can be used once;

[0027] ⑵ Detection and recording

[0028] The piercing potentiometer recording begins. The output signal was recorded on the computer hard disk through an analog-to-digita...

Embodiment 2

[0037] The operating conditions are the same as in Example 1, except that the cotton strains to be tested are B1 and B2 respectively.

[0038] The test results show that for the B1 and B2 cotton lines, the average duration of each occurrence of the waveform corresponding to the mouth needle of the cotton aphid sucking in the phloem of the cotton leaf is 7.99 minutes and 22.99 minutes respectively, while the waveform corresponding to the mouth needle of the cotton aphid sucking in the phloem of the cotton leaf appears The percentages of the sum of time in the determination time are 9.93% and 40.45% respectively;

[0039] According to the evaluation standard, for the B1 strain, the average duration of each occurrence of the waveform corresponding to the mouth needle of the cotton aphid sucking in the phloem of the cotton leaf is 7.99 minutes, which is less than 10 minutes, and the time for the waveform corresponding to the mouth needle of the cotton aphid to suck in the phloem of...

Embodiment 3

[0043] The operating conditions are the same as in Example 1, except that the cotton strain to be tested is insect-resistant transgenic cotton SGK321. The test results show that the average duration of each occurrence of the waveform corresponding to the stylet of cotton aphid sucking in the phloem of SGK321 cotton leaves is 31.68 minutes, and the sum of the corresponding waveforms of the stylet of cotton aphid sucking in the phloem of SGK321 cotton leaves accounts for the average percentage of the measurement time 30.16%;

[0044] According to the evaluation standard, for the SGK321 cotton strain, the average duration of each occurrence of the waveform corresponding to the mouth needle of cotton aphid sucking in the phloem of cotton leaves is 31.68 minutes, which is greater than 14 minutes, and the corresponding waveform of cotton aphid mouth needle sucking in the phloem of cotton leaves appears The average percentage of the sum of time in the total measurement time is 30.16%...

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Abstract

The invention discloses a method for rapidly evaluating an aphid resistance effect of transgenic insect-resistant cotton. A detecting instrument is an insect piercing and sucking potential instrument. The method comprises the following steps of: connecting cotton aphids with a gold wire electrode; detecting and recording electric signals generated when the cotton aphids pierce and suck for feeding on the cotton to be detected; finishing after recording for a certain time, and analysing waveforms generated when the cotton aphids pierce and suck for feeding on the measured cotton; and evaluating the aphid resistance effect of the transgenic insect-resistant cotton on the cotton aphids, wherein if an average value of the duration time of the corresponding waveforms when cotton aphid stylets suck at phloems of cotton leaves each time is less than 10 minutes and the percentage of the time sum of the corresponding waveforms when the cotton aphid stylets suck at the phloems of the cotton leaves in the total measuring time is less than 12 percent, the strain of transgenic insect-resistant cotton has high aphid resistance, and if the average value of the duration time of the corresponding waveforms when cotton aphid stylets suck at the phloems of the cotton leaves each time is more than 14 minutes and the percentage of the time sum of the corresponding waveforms when the cotton aphid stylets suck at the phloems of the cotton leaves in the total measuring time is more than 27 percent, the strain of transgenic insect-resistant cotton has no aphid resistance. Compared with the existing like detection method, the method disclosed by the invention has high detection speed, is simple and has low cost, high repeatability and high accuracy.

Description

[0001] Technical field: [0002] The invention relates to a technology for safety evaluation of transgenic crops, in particular to a method for rapidly evaluating the anti-aphid effect of transgenic insect-resistant cotton. Background technique: [0003] At present, there are mainly the following techniques for evaluating the insect-resistant effect of transgenic insect-resistant cotton: (1) Enveloping aphid test method: inoculate cotton aphids in the enveloping net, and observe the aphid damage index and Aphid damage index decline rate; (2) Aphid weight measurement method: connect cotton aphids to cotton, after a period of feeding, weigh the body weight of cotton aphids, obtain the average single aphid weight, and use this as an index to evaluate the impact of cotton on cotton. The influence of aphid growth and development; (3) Indoor life table method: cotton or cotton leaves are used to feed cotton aphids, and the nymph mortality, nymph duration, adult aphid duration,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00
Inventor 闫凤鸣汤清波崔金杰马英贾述娟雒珺瑜蒋金炜郭线茹周琳高佳
Owner HENAN AGRICULTURAL UNIVERSITY
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