Polyculture enrichment screening method for ocean heterotrophic denitrifying bacteria
A technology for denitrification bacteria and screening methods, applied in the direction of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve problems such as low specific growth rate, many types of microorganisms, and large footprint
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Embodiment 1
[0014] (1), autotrophic enrichment domestication of marine heterotrophic denitrification bacteria: the autotrophic enrichment domestication of marine heterotrophic denitrification bacteria is carried out in a container with a certain volume, and a certain volume of marine aquaculture wastewater is added to the container to control the water body. Dissolved oxygen is above 3mg / L, use Na 2 CO 3 The pH of the solution is adjusted between 7.5-9.0, the salinity is adjusted at 2.5%-3.0%, the temperature of the water body is controlled at 25±2°C, and inorganic ammonia nitrogen is added every day to make the concentration of ammonia nitrogen in the enrichment and acclimatization solution 0.2-15mg / L. After 30 days of enrichment and acclimatization, the added concentration of ammonia nitrogen reached about 10 mg / L, and the autotrophic enrichment and acclimatization stage ended.
[0015] (2) The stage of heterotrophic enrichment and domestication of marine heterotrophic denitrification ...
Embodiment 2
[0021] (1), autotrophic enrichment domestication of marine heterotrophic denitrification bacteria: the autotrophic enrichment domestication of marine heterotrophic denitrification bacteria is carried out in a container with a certain volume, and a certain volume of marine aquaculture wastewater is added to the container to control the water body. Dissolved oxygen is above 4mg / L, use Na 2 CO 3 The pH of the solution is adjusted between 7.0-8.5, the salinity is adjusted at 2.0%-3.5%, the temperature of the water body is controlled at 28±2°C, and inorganic ammonia nitrogen is added every day to make the concentration of ammonia nitrogen in the enrichment and acclimatization solution 0.5-20mg / L. After 50 days of enrichment and acclimatization, the added concentration of ammonia nitrogen reached about 20 mg / L, and the autotrophic enrichment and acclimatization stage ended.
[0022] (2) The stage of heterotrophic enrichment and domestication of marine heterotrophic denitrification ...
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