Chemoluminescence enhancing reagent, using method thereof and chemiluminescent liquid

A chemiluminescence and reagent technology, which is applied in the field of chemiluminescence immunoassay, can solve the problems of high price and difficult purchase, and achieve the effects of long duration, stable luminescence signal and enhanced luminescence signal

Active Publication Date: 2015-01-07
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The polymers and long-chain alkyl fluorescent substances used in the luminescence enhancers of 1,2-dioxetane compounds disclosed in the above patents are expensive and difficult to purchase

Method used

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  • Chemoluminescence enhancing reagent, using method thereof and chemiluminescent liquid
  • Chemoluminescence enhancing reagent, using method thereof and chemiluminescent liquid
  • Chemoluminescence enhancing reagent, using method thereof and chemiluminescent liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～14

[0085] Use ultrapure water to prepare a basic luminescence solution containing the following components:

[0086]

[0087]

[0088] Weigh an appropriate amount of components except AMPPD, dissolve in ultrapure water, adjust the pH value, constant volume, add AMPPD to dissolve, and obtain the basic luminescent liquid with the final concentration of each component as shown in the above table. The chemiluminescent liquids of Examples 1-14 were prepared by adding various enhancer components into the basic luminescent liquid (see Table 1).

[0089] Take 50ul of 1ng / mL alkaline phosphatase solution as a sample, add 100uL of the chemiluminescent solution of each embodiment respectively, measure the chemiluminescent signal on a chemiluminescent detector, and calculate the enhancement factor of the signal value of each luminescent solution and the signal value of the basic luminescent solution. As can be seen from the results in Table 1, only the use of alkyl diquaternary ammoniu...

Embodiment 18

[0094] With reference to the method of Example 1, the chemiluminescent liquid containing the following components was prepared with ultrapure water:

[0095]

[0096] Take 50ul of 1ng / mL alkaline phosphatase solution as a sample, add 100uL of the chemiluminescence solution of this example, measure the chemiluminescence signal on a chemiluminescence detector, and draw a time curve of the luminescence reaction. The result is as figure 1 As shown, the luminescent signal of the chemiluminescent liquid disclosed in the present invention is stable and lasts for a long time.

Embodiment 19

[0097] Embodiment 19 (AMPPD+ enhancer)

[0098] With reference to the method of Example 1, the chemiluminescent liquid containing the following components was prepared with ultrapure water:

[0099]

[0100] Take 20ul 1ng / mL alkaline phosphatase solution as the sample, add 100uL luminescence solution, measure the chemiluminescence signal on a chemiluminescence detector, and compare it with the luminescence signal of the commercial luminescence solution Access Substrate (Beckman). The result is as figure 2 As shown, under the action of the enhancer of the present invention, the luminescent signal of AMPPD is greatly enhanced, and the performance of the enhanced luminescent liquid is close to that of Access Substrate (Beckman).

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Abstract

An enhancing reagent for enhancing chemiluminescence of 1,2-dioxetane compounds and a method for using the enhancing reagent to enhance the chemiluminescence are provided, in which the enhancing reagent contains an alkyl bis-quaternary ammonium salt of Formula I. A chemiluminescent composition with a 1,2-dioxetane compound as a substrate and a kit thereof are further provided, which contain a 1,2-dioxetane compound and an alkyl bis-quaternary ammonium salt of Formula I.

Description

technical field [0001] The present invention relates to chemiluminescent immunoassays. More specifically, the present invention relates to a chemiluminescence liquid containing an enhancer, a preparation method and a kit; a reagent and a method for enhancing the chemiluminescence of 1,2-dioxetane compounds, a preparation method and a kit. Background technique [0002] Chemiluminescence immunoassay is a non-radioactive immunoassay technology that develops very rapidly in the world. It is an ultra-high sensitivity trace detection technology developed after enzyme immunoassay, radioimmunoassay and fluorescence immunoassay. It has the advantages of high sensitivity, wide detection range, simple and fast operation, good stability of markers, and no pollution. It is currently the most ideal method for immunoquantitative analysis. [0003] In chemiluminescent immunoassays, commonly used luminescent substances include luminol, isoluminol, acridinium esters, and 1,2-dioxetane compou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/07G01N33/53G01N21/76
CPCG01N33/532G01N33/542C09K11/07G01N33/58
Inventor 钱纯亘张裕平
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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