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Gingko leaf composition and preparation method thereof

A composition, technology of ginkgo biloba, applied in the field of medical invention, can solve problems such as lactone substance damage, achieve the effects of avoiding pollution, stable content of active ingredients, and high yield

Active Publication Date: 2014-05-14
HEILONGJIANG ZBD PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adjusting the alkalinity before putting on the resin column is to make the flavonoids easy to adsorb on the macroporous adsorption resin column, but it will destroy the lactones

Method used

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  • Gingko leaf composition and preparation method thereof
  • Gingko leaf composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: Ginkgo biloba composition

[0057] 1. Weigh 1kg of ginkgo leaves, moderately crush (below 0.5cm), and reflux extraction twice. For the first time, add 5 times the amount of 65% ethanol to soak for 2 hours, and reflux for 2 hours; add 4 times the amount of 65% ethanol for the second time , reflux for extraction for 1.5 hours, combine the extracts, and set aside;

[0058] 2. Adjust the pH value of the extract to 7.6 at 35°C, let it stand for 12 hours, filter, recover ethanol until it has no alcohol smell; then add water to dilute to 2g / ml of crude drug, adjust the pH value to 5.0, centrifuge, and apply HPD450 to the centrifuge Macroporous adsorption resin column, wash the resin column with 1 times of resin purified water, 2 times of resin with sodium acetate solution with a concentration of 3.0% (g / ml), then rinse the resin column with purified water until the pH of the washing solution is neutral , then rinse the resin column with 1.5 times the concentrati...

Embodiment 2

[0106] Embodiment 2: Ginkgo biloba composition

[0107] 1. Weigh 1 kg of ginkgo leaves, moderately crush (below 0.3cm), reflux extraction twice, first add 6 times the amount of 80% ethanol to soak for 1.5 hours, reflux extraction for 1.5 hours, add 3 times the amount of 80% ethanol for the second time Reflux extraction for 1 hour, combine the extracts, and set aside;

[0108] 2. Adjust the pH value of the extract to 7.9 at 33°C, let it stand for 18 hours, filter, recover ethanol until it has no alcohol smell, then add water to dilute to 2.1g / ml crude drug, adjust the pH value to 4.0, centrifuge, and put the centrifuge on the Macroporous adsorption resin column (model is HPD450), wash the resin column with 1 times of resin in purified water, 2 times of resin in sodium acetate solution with a concentration of 3.2% (g / ml), and then wash the resin column with purified water until it is washed solution pH 7.0, then wash the resin column with 20% ethanol with a concentration of 1.5...

Embodiment 3

[0111] Embodiment 3: Ginkgo biloba composition

[0112] 1. Weigh 1 kg of ginkgo leaves, moderately crush (less than 0.2 cm), reflux extraction twice, first add 4 times the amount of 75% ethanol to soak for 2 hours, reflux extraction for 2 hours, add 5 times the amount of 70% ethanol for the second time Reflux extraction for 1.5 hours, combine the extracts, and set aside;

[0113] 2. Adjust the pH value of the extract to 7.0 at 37°C, let it stand for 24 hours, filter, recover the ethanol until it has no alcohol smell, then add water to dilute to 1.8g / ml of crude drug, adjust the pH value to 4.5, centrifuge, and put the centrifugal liquid on the Macroporous adsorption resin column, wash the resin column with purified water of 1 times the amount of resin, sodium acetate solution of 2.9% (g / ml) in the concentration of 2 times the amount of resin, and then wash the resin column with purified water until the pH of the washing solution is 7.0 , then rinse the resin column with 20% e...

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Abstract

The invention relates to a gingko leaf composition and a preparation method thereof. The composition comprises the following components: 24 to 45 percent of total flavonol glycoside, 4 to 18 percent of ginkgolide, 2 to 6 percent of bilobalide, and less than 5 ppm of ginkgoic acid. The invention provides a simple, feasible and reliable method, wherein total flavonoid and terpene lactones in a certain proportion can be obtained simultaneously in the extraction process the yield is high, purity of effective components is high and fewer impurities exist, and harmful substances such as alkylphenolic acid can be effectively removed.

Description

technical field [0001] The invention relates to the field of medical inventions, in particular to a ginkgo biloba composition and its preparation method and application. Background technique [0002] Ginkgo biloba is a perennial deciduous tree of the Ginkgo family Ginkgo family. It has existed on the earth for more than 200 million years and is known as "natural living fossil". [0003] Ginkgo biloba is also known as Gongsun tree, ginkgo tree, etc. Its seeds, ginkgo, have a long history in my country as a traditional Chinese medicine for relieving cough, relieving asthma, reducing stool, and stopping turbidity. Its leaves, Ginkgo biloba, also known as ginkgo leaves, are bitter, sweet and astringent, flat in nature and slightly poisonous. The chemical composition of Ginkgo biloba is very complex. More than 170 compounds have been found so far, mainly flavonoids, terpenes, alcohols, alkaloids, polyisoprenes, polysaccharides and other active ingredients with pharmacological ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/16A61P9/10A61P9/14A61P27/02A61K127/00
Inventor 方同华
Owner HEILONGJIANG ZBD PHARMA
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