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A method and kit for detecting the content of monoamine oxidase

A technology of monoamine oxidase and detection method, which is applied in the field of medical inspection and measurement, can solve the problems of poor stability of hydrogen peroxide and reduced accuracy of detection results, and achieve the effects of good stability, long storage period and improved accuracy

Inactive Publication Date: 2011-11-30
董理
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above-mentioned determination method needs to measure the concentration of pyruvic acid through the hydrogen peroxide route, and the stability of hydrogen peroxide is poor, which reduces the accuracy of the detection results.

Method used

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  • A method and kit for detecting the content of monoamine oxidase
  • A method and kit for detecting the content of monoamine oxidase
  • A method and kit for detecting the content of monoamine oxidase

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Embodiment 1

[0038] Embodiment 1: The kit for detecting monoamine oxidase of the present invention

[0039] The kit for detecting monoamine oxidase in the present invention is a liquid single reagent, including: 100mmol / L Tris-HCl buffer solution at pH 8.8-9.0, 18mmol / L amine compound, 1.5mmol / L reduced coenzyme, 14mmol / L α-ketone Glutaric acid, 1500U / L glutamate dehydrogenase and 3000U / L lactate dehydrogenase.

Embodiment 2

[0040] Embodiment 2: The kit for detecting monoamine oxidase of the present invention

[0041] The kit for detecting monoamine oxidase of the present invention is a double reagent, the first reagent includes 80mmol / L Tris-HCl buffer solution of pH9.1~9.2, 10mmol / L α-ketoglutarate, 15mmol / L amine compound, the second reagent Including 50mmol / L Tris-HCl buffer solution with pH8.6~8.8, 1.0mmol / L reduced coenzyme, 2000U / L glutamate dehydrogenase and 4000U / L lactate dehydrogenase.

Embodiment 3

[0042] Embodiment 3: the kit for detecting monoamine oxidase of the present invention

[0043] The kit for detecting monoamine oxidase in the present invention is a liquid single reagent, including: 200mmol / L Tris-HCl buffer solution of pH 8.8-9.0, 20mmol / L amine compound, 1.5mmol / L reduced coenzyme, 20mmol / L α-ketone Glutaric acid, 3000U / L glutamate dehydrogenase and 5000U / L lactate dehydrogenase.

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Abstract

The invention relates to the technical field of medical testing and determination, and discloses a method and a kit for detecting monoamine oxidase. The method for detecting monoamine oxidase provided by the invention does not go through the hydrogen oxidation route, but uses the action of glutamic acid dehydrogenase to measure the decline rate of the reduced coenzyme reacting with ammonia to calculate the content of monoamine oxidase, thereby improving the accuracy of the detection result. The detection method of the present invention also includes mixing excessive lactate dehydrogenase with the sample to be tested, eliminating endogenous pyruvate in the sample during the reaction delay period, so that the reduced coenzyme reaches a balance, and the accuracy of monoamine oxidase detection is improved. The kit of the invention has good stability and long storage period, and the detection result by using the kit of the invention has high accuracy, good precision and wide linear range. The kit of the invention has a wide application range and is convenient for popularization and use, and can be applied to hospitals at all levels, health prevention departments and medical and biological scientific research units to determine the content of monoamine oxidase in serum.

Description

technical field [0001] The invention relates to the technical field of medical testing and determination, in particular to a method and kit for detecting the content of monoamine oxidase. Background technique [0002] Monoamine oxidase (monoamine oxidase, MAO) is an enzyme that catalyzes the oxidative deamination of monoamine. Also known as flavinamine-containing oxidase, an enzyme that inactivates catecholamine neurotransmitters and is used as an antidepressant drug. The activity of serum monoamine oxidase can reflect the degree of liver fibrosis and is an important indicator for the diagnosis of liver cirrhosis. The positive rate of elevated serum monoamine oxidase activity in patients with liver cirrhosis is more than 80%, and the highest value can exceed twice the reference value of the control, and the increase in serum monoamine oxidase activity is parallel to the process of liver surface nodule formation. Serum monoamine oxidase activity does not increase in patient...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 董理
Owner 董理
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